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Experimental Study on Muscle Effected by Glucocorticoid

Author: ZhangZheng
Tutor: WangPei；SuLianZuo
School: Tianjin Medical University
Course: Surgery
Keywords: Glucocorticoids Gluteus Amyotrophic Muscle fiber Electron density
CLC: R595
Type: Master's thesis
Year: 2008
Downloads: 42
Quote: 0
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Abstract


Objective: glucocorticoid synthesis and secretion by the adrenal cortex, involved in the body's stress, immune regulation, multifaceted role against invasive pathogens and anti-inflammatory. Glucocorticoids is a widely used clinical drug orthopedic topical steroid application is mainly found in the closed treatment. Because the operation is simple and quick, broad clinical application. During the operation, it is inevitable to extravasation to muscle tissue, resulting in side effects. Less local steroid injections cause muscle damage study is currently limited to case reports (local muscle atrophy). Experimental study of the effects of local steroid injection of the muscle not been reported, due to glucocorticoid-induced skeletal muscle atrophy mechanism is not fully understood, in New Zealand white rabbits as experimental animals explore the morphology of glucocorticoids on muscle tissue the impact. METHODS: The experiment 2007-06/2007-11 Tianjin Medical University General Hospital Laboratory. 24 healthy adult New Zealand white rabbits were randomly divided into 2 groups, 12 in each group, the experimental group, the normal group. Experimental group self-control, each animal experiment side to the side of the gluteus maximus, the contralateral gluteus to control side. Normal group experimental side to the side of the gluteus maximus, the contralateral gluteus to control. Experimental group were injected glucocorticoid normal group was injected with the appropriate dose of saline. And take the corresponding part of the muscle tissue biopsy hematoxylin - eosin staining, light microscopy and electron microscopy and image analysis. Image-pro-plus image analysis system is stored in the computer by the camera after the measured maximum cell diameter of the unit area within the muscle cells and muscle cell number. Application SPSS11.5 statistical software for data statistical processing, using the t test for two independent sets of sample data. Results: Inclusion 24 animals entered the final analysis. ① histological findings: paraffin-embedded sections of hematoxylin - eosin staining observed experimental side of the muscle fibers arranged in neat rows, the integrity of the muscle cell, no degeneration and necrosis of the nucleus in the edge of the muscle cells sarcomere integrity. The cross-section of muscle cells the diameter significantly reduce the number of muscle cells in the unit area increased significantly. After statistical tests to find themselves against the side of the muscle fiber size, number in the experiment with intramuscular side muscle fibers difference was not statistically significant. The maximum diameter and the number of cells the difference was statistically significant group of normal muscle cells. ② electron microscope view of the findings: normal rabbit gluteus maximus muscle fibers mixed I and type II muscle fibers alternating type I fibers less than type Ⅱ fibers. The observations indicate that the experimental side and the control side, type II fibers significantly reduced the electron density of type II muscle fibers than type I muscle fibrils, and uneven electron density of type II muscle fibers arranged in distorted, no significant change in type I muscle fibers. There are significant differences in the muscle fibers of the experimental group and the normal group of muscle fibers. Conclusion: glucocorticoid sure induced muscle atrophy, no statistically significant difference between effects of intramuscular local and systemic muscle tissue. The main impact of type II muscle fibers of the muscle tissue, no significant impact on the type I muscle fibers.

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