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Objective 1. Acute myocardial infarction in patients with circulating endothelial progenitor cells (EPCs) CD34 ~ KDR ~ correlation levels of aldehyde dehydrogenase 2 (ALDH2) gene polymorphism. 2 evaluation of inflammatory factors, high-sensitivity C-reactive protein (hs-CRP) in which the role. Methods A case-control study was conducted. Selected study case group in March 2007 to January 2008, Qilu Hospital of Shandong University, admitted to acute myocardial infarction in 101 patients and the healthy control group of 30 patients. Questionnaire record all clinical study data, including age, sex, smoking, hypertension, diabetes, lipid levels, family history of cardiovascular disease and other projects in the form of in which lipids projects including total cholesterol (Total cholesterol, TC), glycerol triester (triglyeride, TG), LDL (low density lipoprotein-cholesterol, LDL-C), high-density lipoprotein (high density lipoprotein-cholesterol, HDL-C). Take myocardial infarction in patients with peripheral venous blood was measured by flow cytometry, circulating EPCs CD34 ~ KDR ~ level of immune transmission Turbidimetric Determination of serum hs-CRP concentration, and polymerase chain reaction (polymerase chain reaction, PCR) and direct sequencing analysis of the ALDH2 gene polymorphism. Statistical analysis using SPSS software, as the level of circulating EPCs with traditional cardiovascular disease risk factors and ALDH2 gene polymorphism analysis, and multiple linear regression model analysis of the evaluation inflammatory cytokines hs-CRP in patients with acute myocardial infarction the role played by the relationship between circulating of EPCs levels of ALDH2 gene polymorphisms. Results in patients with acute myocardial infarction, the level of circulating EPCs in smoking, mutant ALDH2 gene (ALDH2 * 1 / * 2 ALDH2 * 2 / * 2), diabetes was significantly lower than non-smokers, the wild-type ALDH2 gene (ALDH2 * 1 / * 1), diabetes (P <0.05), and LDL-C concentration EPCs level showed a significant negative correlation (R = -0.357, P <0.05). Acute myocardial infarction Circulating EPCs, hs-CRP levels significantly higher than normal control group (P <0.001) and hs-CRP concentration and EPCs level showed a significant positive correlation (R = 0.631, P <0.001), while the mutant the ALDH2 gene distribution frequency between the two groups there was no significant statistical difference. 3. Acute myocardial infarction, wild-type ALDH2 gene by circulating EPCs, hs-CRP levels significantly higher than the mutation genotype (P <0.01). In all patients with acute myocardial infarction using multiple linear regression analysis, regression model ALDH2 genotype, age, gender, smoking, hypertension, diabetes, family history of cardiovascular disease, LDL-C, HDL-C, TC TG as independent variables. As the dependent variable, the level of circulating EPCs mutant ALDH2 gene, smoking, LDL-C risk factors that affect the level of circulating EPCs, Beta value of -0.349, -0.255, -0.218, respectively, in the regression analysis, hs-CRP variables, the correlation weakened mutant ALDH2 genotype and the level of circulating EPCs, Beta = -0.174, P = 0.037, and hs-CRP level of circulating EPCs (Beta = 0.329, P = 0.013); to hs-CRP levels as the dependent variable, the correlation found between mutant ALDH2 gene and hs-CRP (Beta = -0.198, P = 0.029). Conclusion 1. Acute myocardial infarction in patients with the level of circulating EPCs is associated with traditional cardiovascular risk factors. The number of circulating EPCs in acute myocardial infarction, inflammatory factors hs-CRP concentration increased significantly, and both showed a positive correlation, hs-CRP may be involved in the bone marrow EPCs mobilization. Mutant ALDH2 gene in patients with acute myocardial infarction circulating EPCs, hs-CRP levels of risk factors and hs-CRP may play an intermediary role. Tip mutant ALDH2 gene can be used as indicators of poor prognosis of myocardial infarction.
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