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Effects of Early Rehabilitative Training on Brain Structure and Learning Function Following Hypoxic-Ischemic Insult in Neonatal Rats

Author: HuZhanQi
Tutor: JiangLi
School: Chongqing Medical University
Course: Pediatrics
Keywords: Hypoxic-ischemic brain injury Early rehabilitation Synaptophysin Brain-derived neurotrophic factor TUNEL
CLC: R49
Type: Master's thesis
Year: 2008
Downloads: 113
Quote: 0
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Abstract


The first part of perinatal hypoxic-ischemic brain injury model Objective: To establish the neonatal rat perinatal hypoxic-ischemic brain injury model. Methods: 7-day-old SD rats were randomly divided into sham operation group and hypoxic-ischemic model group (HIBD). After the the HIBD group of rats were anesthetized ligation of the left common carotid artery, placed in the hypoxic chamber continued to pass into the 8% of the nitrogen-oxygen mixture 2.5h; sham-operated group, that is, only free without ligation of the left common carotid artery, not hypoxia. 72h after the injury the HE staining pathological changes of brain tissue water maze learning and memory of rats in the full 30-day-old rats. Results: Compared with the normal control group, HIBD rats modeling side of the brain tissue volume increased edema; HE staining light microscopy examination showed a large number of neuron degeneration and necrosis, manifested as increased cell volume, severe cases, the nucleus disappeared and a large number of neurons disappear \Conclusion: In this study, the methods used to establish newborn rats perinatal hypoxic-ischemic brain damage model and clinical similarity can be related to research. The second part of early rehabilitation training on hypoxic-ischemic brain structure and cognitive function in rats Objective: To investigate the impact of early rehabilitation of hypoxic-ischemic brain damage (HIBD) in rat brain structure and cognitive function. Methods: 24-day-old SD rats were randomly divided into normal control group, HIBD group, HIBD rehabilitation intervention group. Rice method, the the HIBD group and HIBD rehabilitation intervention group establish HIBD model, the HIBD rehabilitation 72 hours to begin a 30-day early rehabilitation intervention group was modeled. After the end of the experiment, learning and memory by Morris water maze test, the observed changes in brain weight, HE staining hippocampal and cortical neuron disease. TUNEL method the observed neuronal apoptosis in the brain; Immunohistochemistry was used to detect the expression of synaptophysin (SYN) and brain-derived neurotrophic factor (BDNF). Results: (1) HIBD rats learning and memory retention were significantly lower than the the HIBD rehabilitation intervention group and normal control group, the HIBD rehabilitation intervention group test scores compared with the control group no significant difference. (2) the HIBD group and HIBD rehabilitation intervention group modeling side of the brain weight were significantly lower than the control group (0.638 ± 0.019), the HIBD rehabilitation intervention group modeling side of the brain (0.428 ± 0.085g) was significantly higher than the HIBD (0.348 ± 0.043g). The three groups of rats modeling side brain weight was no significant difference. (3) the HIBD group and HIBD rehabilitation intervention group rat brain cortex and hippocampus group of surviving neurons than the normal control group. But the HIBD group, HIBD rehabilitation intervention group surviving neurons number increased significantly. (4) the HIBD group and HIBD rehabilitation intervention group rat brain frontal cortex and hippocampal CA1 region of apoptotic cells than normal controls group. But compared with the HIBD, HIBD rehabilitation intervention group apoptotic cells decreased significantly. (5) HIBD hippocampal CA1 region of synaptophysin optical density value was significantly lower than that in normal control group and HIBD rehabilitation intervention group, and no significant difference between the the HIBD rehabilitation intervention group and normal control group. (6) HIBD CA1 region of hippocampus and frontal cortex BDNF optical density was significantly lower than that in normal control group and HIBD rehabilitation intervention group, and no significant difference between the the HIBD rehabilitation intervention group and normal control group. Conclusion: Early rehabilitation training on the the perinatal HIBD brain structure and cognitive function recovery has a certain role to reduce the HIBD brain tissue of neuron apoptosis and promote survival of nerve cells in the brain after injury, promote the reconstruction of synaptic, enhanced BDNF The expression, thus contributing to brain injury and repair.

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