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Objective: To explore the high-volume hemofiltration of septic shock canine lung tissue TLR4mRNA expression of. Methods: 24 of the mongrel male dogs were randomly divided into three groups, each group of eight, including mechanical ventilation of the normal control group underwent sham treatment; the control group and the treatment group were confirmed by central venous 30min injection of endotoxin (LPS) 650μg/kg induced septic shock model in dogs: control group underwent sham treatment of mechanical ventilation, mechanical ventilation treatment group high-capacity hemofiltration treatment. Monitoring base and into a mold (0h) HR, MAP, PAP, CVP, PAWP, CO values ??calculated PVR, SVR value; measured by radioimmunoassay in the control group, the treatment group treatment 0h, 1h, 2h, 4h circulating blood TNF-α, IL-6, IL-10 the concentration; treatment for 4h, whichever lung thoracotomy and placed in liquid nitrogen flask save by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) method for the determination of each group TLR4mRNA expression levels. Results: 1, intravenous injection of LPS, MAP, CO, HR gradually decreased gradually increased PAP, PAWP, CVP, PVR, SVR, that the model established when the map than the base value decreased by 20% or more. Into a mold of each indicator compared with baseline were statistically significant differences (P lt; 0.01) 2, was measured by radioimmunoassay treatment group after 1h circulating blood TNF-alpha, IL-6, IL-10 concentration is decreased, and the continuous downward trend (P lt; 0.01). Indicators and treatment 0h of the control group difference was not significant (P gt; 0.05) Comparing the two groups, the treatment group at each time point were lower than the control group (P lt; 0.01). And TLR4mRNA expressed in the normal control group, the control group, the treatment group were 0.895 ± 0.048,0.987 ± 0.036,0.931 ± 0.019, the TLR4mRNA of the control group compared with the control group were significantly increased (P lt; 0.01); treatment group compared with the control group TLR4mRNA expression lowered (P lt; 0.01). The correlation analysis showed that expression of the circulating blood of TLR4mRNA in TNF-alpha, IL-6, IL-10 concentration was positively correlated (P lt; 0.05) correlation coefficients were: 0.821,0.542,0.610. Conclusion: The normal canine lung tissue TLR4 expression; bacterial endotoxin LPS raised septic shock canine lung tissue TLR4 mRNA expression, and promote the release of inflammatory mediators; HVHF down the septic shock dog TLR4mRNA of the expression, reduce inflammatory response. The mechanism may be removed by convection, adsorption of inflammatory mediators weaken the inflammatory mediators of TLR4mRNA positive feedback regulation; stable environment, eliminating the stress response, downward TLR4mRNA on. High volume hemofiltration regulation of TLR4 may provide a new theoretical basis for the treatment of septic shock.
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