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Research on the Glucose Regulating ANGPTL3 Expression and the Mechanism in L02

Author: ZengJianTao
Tutor: WangJiHong
School: Chongqing Medical University
Course: Biochemistry and Molecular Biology
Keywords: angiopoietin-likeprotein 3 Liver X receptor metabolic syndrome
CLC: R341
Type: Master's thesis
Year: 2008
Downloads: 37
Quote: 0
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Abstract


Objective To observe the effects of glucose on hydroxyl-methyl -glutaryl coenzyme A reductase (HMGCR)、Liver X receptorα(LXRα) and angiopoientin-like protein 3(ANGPTL3)expression in human liver cell(L02). To investigate the effects of simvastatin on those genes in different glucose concentration. In order to explore the pathway by which glucose effect the expression of ANGPTL3 and the significance of ANGPTL3 in metabolic syndrome.Methods L02 cells were cultured in mediums containing different concentration with simvastatin and without simvastatin separately.The relative level of HMGCR、LXRαand ANGPTL3 mRNA expression were detected by reverse transcription-polymerase chain reaction(RT-PCR). The relative level of ANGPTL3 protein was measured by western blotting. Quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme link assays.Results In the group without simvastatin, The contents of total cholesterol increased significantly in L02 cell, and had significant difference (P<0.05).The expression of LXRα、ANGPTL3 mRNA and ANGPTL3 protein also increased with the increasing of the glucose concentration(P<0.05). In the group which contain simvastatin, The expression of LXRα、HMGCR、ANGPTL3 mRNA and ANGPTL3 protein decreased comparing to the group without simvastatin.Conclusions Glucose can increase total cholesterol , upregulate the expression of LXRαand ANGPTL3 in L02 cell. Simvastatin can inhibit the upregulation.Glucose may interfere ANGPTL3 mRNA through incresasing total cholesterol , activing LXRα. The activation of LXRαupregulates the expression of ANGPTL3. LXRαand ANGPTL3 may be the association between diabetes mellitus and lipid metabolism disorder.

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