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Skullcap antipyretic pharmacodynamic differences comparison based on different sources and Mechanism

Author: XuShan
Tutor: MengQingGang
School: Beijing University of Traditional Chinese Medicine
Course: Basic Theory of Traditional Chinese Medicine
Keywords: Scutellaria Different sources Antipyretic pharmacodynamic Mechanism of action
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 277
Quote: 0
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Abstract

Labiatae skullcap (Scutellaria baicalensis Georg) dried root, skullcap (Radix Scutellariae), only contained in the \This product is commonly used in clinical Qingre Chinese medicine, applications historic medicinal great demand for one of the large species of Chinese herbal medicines in China, and has good prospects for development. Consolidated in recent years reported in the literature found for the study of the mechanism of the skullcap antipyretic effect, confined to a single active ingredient - the study of the antipyretic mechanism of baicalin and wild Scutellaria glycosides (Scutellaria stem and leaf extract) for skullcap common mechanism of action of a variety of active ingredients in the extract is less difficult to reflect the the antipyretic effect mechanism skullcap as a whole medicine. Moreover, its antipyretic mechanism is also more concentrated in the body Wenzhong Shu positive regulation of the media, but for the the induced thermal cytokines and Wenzhong Shu negative regulator of the media's role has not been reported. Moreover, skullcap, from different sources, the main active ingredient content of trace elements there are differences. As authentic herbs of Hebei Rehe yellow baicalensis its yellow baicalensis glycoside content of less than Shanxi, Shandong region of yellow baicalensis, but its in antipyretic effect is better than other parts of the yellow baicalensis; and cultivation skullcap of baicalin content higher, but clinical admits wild skullcap efficacy. Therefore, the content of baicalin can not be fully representative of the efficacy quality skullcap skullcap other active ingredients, in the role of certain pharmacodynamic (such as fever), may play a key role. It showed as the the skullcap quality testing standards are doubtful baicalin content. Therefore, this subject Dalian City Technology major project \) as the basis, based on the theory of TCM, the main research method Pharmacology, skullcap basis of efficacy - heat purging fire detoxification, carry antipyretic pharmacodynamic test, the most common forms of medicine from the medicine - aqueous extract of Scutellaria start skullcap as a whole medicine antipyretic mechanism; compare different sources skullcap (Hebei in Dalian, Shanxi) antipyretic effect pharmacodynamic differences, combined with traditional Chinese medicine fingerprints to identify solutions spectrum efficiency of the thermal effect relationship. The experiment is divided into two parts: the experimental skullcap to extract matter antipyretic mechanism research purposes from the most common herbs used as medicine in the form - water extract of Scutellaria start from pyrogenic cytokines body Wenzhong Shu positive regulatory media body Wenzhong Shu negative adjust the angle of the medium, and efforts to make a comprehensive study of the antipyretic effect mechanism skullcap medicine as a whole showed. Method 1 dry yeast induced rat fever model of male Wistar rats were used as experimental animals, aspirin, dexamethasone used as a positive control drug. Qualified rats were randomly divided into seven groups, n = 8, respectively, for the Dalian skullcap water extract large, low-dose group (group 3), and the normal control group, model group, aspirin positive control group and dexamethasone positive control group. Gavage for the first time after 1 hour, each group with subcutaneous injection of 20% yeast suspension (normal control group injected with sterile saline). Fever peak of the first eight hours after modeling to measure the body temperature of rats. 2 Observation items detection method maximum temperature rise in the height (ΔT / ° C): calculate the maximum increase in body temperature, the temperature maximum difference between the basal body temperature. Serum IL-1β: Experiment 8 hours, immediately decapitated and the animals, take the trunk blood, centrifuged supernatant sample determination by radioimmunoassay kit instructions. CAMP and IL-1β in the hypothalamus: Experiment After 8 hours, immediately decapitated animals, the whole brain quickly removed, under ice-cooling in the suprachiasmatic and gray nodules removed hypothalamus, put into liquid nitrogen fixation after 20 minutes, transferred to -86 ℃ refrigerator analyte. Determination of the sample by radioimmunoassay kit instructions. Brain ventral septal area (Ventral septal Area, VSAs) of AVP: the Experiment 8 hours immediately after the decapitated animals, whole brain quickly removed under ice taken VSA brain tissue, into fixed in liquid nitrogen for 20 minutes, transferred to -86 ℃ refrigerator analyte. The measuring method according to the RIA kit instructions. Results in modeling after 8 hours fever group entering the fever peak of height seen by the maximum temperature rise, high-dose group, aqueous extract of Scutellaria obvious the antipyretic advantage (P lt; 0.05) compared to the small and medium-dose group, and to considerable clinical dose rate the experimental dose aspirin antipyretic effect (P gt; 0.05); aqueous extract of Scutellaria significantly reduced hypothalamic IL-1β and CAMP content (dry yeast induced fever rats with model group P lt; 0.05 ), but also can reduce the ventral septal area of ??brain tissue AVP content (compared to the model group P lt; 0.05) rats largest increase in body height and hypothalamic IL-1β CAMP content and ventral septal area of ??brain The positive correlation between the organization of the content of the AVP (correlation coefficient r were 0.911,0.967,0.853; P lt; 0.05); the dexamethasone group of fever serum IL-1β level significantly reduce the role (compared with model group P lt; 0.05), compared with the normal group, the difference was not significant (P gt; 0.05); inhibition of the fever serum IL-1β content of other drug group does not significantly (compared with the model group P gt; 0.05) . Conclusions of an experimental animal model of dry yeast induced fever successfully copied. The model has the following features: fever stable, long duration, good reproducibility; prone differences in high heat; local inflammation, with the clinical partners visceral or skin acute inflammation in heat syndrome similar. Suitable animal models as a the research clearing - heat herbs skullcap antipyretic pharmacodynamic mechanism. Skullcap antipyretic effects of the aqueous extract showed significant dose-related, high-dose group antipyretic effect optimal clinical dose aspirin antipyretic effect quite the following; the overall aqueous extract of Scutellaria antipyretic one of the mechanisms may : reduce induced hot cytokines IL-1β, to reduce the CAMP the content of the central heating medium, the content in the body Wenzhong Shu promote the hub antipyretic media AVP release. The antipyretic efficacy diff purpose of the experiment two different sources of Scutellaria extract different sources skullcap antipyretic effect pharmacodynamic differences. TCM fingerprint to identify the spectral efficiency of the antipyretic effect relationship, looking for the material basis of the skullcap antipyretic effect. And in future drug active ingredients extracted based on combination, with a different ratio of pharmacodynamic experiment to find the best ratio to lay the foundation for new in-depth study of the skullcap quality testing and innovative drugs experimental basis. Method 1 dry yeast induced rat fever model modeling and method of administration with the former. With aspirin as a positive control drug. Temperature qualified rats were randomly divided into 9 groups of 10 each, respectively, over the aqueous extract of Scutellaria group (group 3), around skullcap alcohol extract group (group 3), normal control group, model group, aspirin positive control group. 1 0.5,1,2,4,6,8,10 and 12 hours each measuring body temperature of rats after modeling the observed temperature changes (the difference between the measurement of body temperature basal body temperature). 2 observation project and detection methods mean body temperature curve: a high degree rise in body temperature (Δt) for the vertical measurement time as abscissa, according to each measurement point number plotted the average body temperature curve. The largest increase in body height (ΔT / ℃): calculate the maximum increase in body temperature, the temperature maximum difference between the basal body temperature. Temperature response index (thermal response index, TRI12): calculate the area between the body temperature curve baseline. Result by the average change in body temperature curve, ΔT and TRI12 can be drawn: dry yeast as exogenous pyrogen model of rat fever fever is caused by relatively stable, successful modeling; different origin skullcap extract (water extraction, alcohol extraction) both showed significant antipyretic effect (P lt; 0.05); during the whole experiment, the the various origin skullcap alcohol extract compared with the same origin of the water extract showed the better antipyretic role (P lt; 0.05) ; various origin of skullcap water mentioning matter of antipyretic role with the clinical dose aspirin role quite (P gt; 0.05); different origins yellow baicalensis extract phase comparison, from in Hebei's baicalin mention material compared to the other two origin antipyretic effects of the alcohol extract advantage (P lt; 0.05). Short compared to the other groups, Hebei the skullcap alcohol extracts showed significant antipyretic effect advantage. Conclusion compare different origin (Hebei, Dalian, Shanxi,) skullcap water extract and ethanol extract of antipyretic pharmacodynamic differences. The results show that the origin for, skullcap alcohol, Hebei extracts obvious antipyretic effect compared with other groups performance advantage. On this basis, combining the experimental results with traditional Chinese medicine fingerprint, to identify the spectrum of different origin skullcap extract antipyretic effect relationship, and ultimately to identify the material basis of Scutellaria antipyretic pharmacodynamic.

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