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Objective: To study kidney yang, the active ingredient in traditional Chinese medicine Epimedium Icariin (ICA) on the proliferation of bone marrow mesenchymal stem cells (MSCs) proliferation and differentiation into cardiomyocytes implications for medicine combined stem cell transplantation treatment of heart failure lay foundation. Methods: Cultured human MSCs, take the third generation of MSCs, by MTT assay icariin (sub 20ug / l, 40ug / l, 80ug / l, 160ug / l four concentrations) on MSCs proliferation. With icariin (ICA), 5 - azacytidine (5-aza) and their combined effects, as differentiation-inducing agents, continuous observation of four weeks, a difference of morphological changes were observed under a microscope, cardiac-specific immunofluorescence staining Troponin I (TroponinI, cTnI), connexin 43 (C0nnexin43, CX43), flow cytometry cardiomyocyte-like cells ratio (ie, cTnI, CX43 percentage of positive cells), semi-quantitative RT-PCR analysis of GATA technology -4, Nkx2-5-related gene transfer empty expression during differentiation, the application of Western-blotting method for analyzing protein level GATA-4, Nkx2-5 expression during differentiation. Results: icariin group absorbance (OD) than the control group (P <0.01), and the concentration of icariin 80ug / l most appropriate. Immunofluorescence results :5-aza-inducing agent group and icariin inducing agent 5-aza group had cTnI and CX43 expression, icariin group cTnI and a weak expression of CX43, while the control group had no expression. Flow cytometry results: Icariin 5-aza group expressed cTnI, CX43 were 47.22%, 44.43% ,5-aza group expressed cTnI, CX43 were 29.10%, 27.15% respectively, there were significant differences (P <0.01), pure icariin group weakly expressed cTnI, CX43 were 7.02%, 7.69% in the control group basically did not express cTnI, CX43. Semi-quantitative RT-PCR results show :5-aza group and icariin 5-aza group in the induction of 28 days, there is a strong expression of GATA-4, Nkx2-5, and 5-aza group icariin expressing GATA- 4, Nkx2-5 compared with 5-aza set of enhancements, Epimedium group weakly expressed GATA-4, Nkx2-5, the control group had no GATA-4, Nkx2-5 expression. Western-blotting showed 5-aza group and icariin induced by 5-aza group after 28 days, there is a strong expression of GATA-4, Nkx2-5, and 5-aza group icariin express GATA-4, Nkx2 -5 compared with 5-aZa set of enhancements, Epimedium group weakly expressed GATA-4, no expression of Nkx2-5, the control group without GATA-4, Nkx2-5 expression. Conclusion: Icariin can promote the proliferation of MSCs. Icariin 5-aza synergistically induce MSCs differentiation into cardiomyocytes, can serve as better promote the differentiation of MSCs into cardiomyocytes conditions. Icariin may induce MSCs differentiation into cardiomyocytes trends, pending further study.
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