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A Study of the Prevention and Cure Effects of Oxymatrine on Periodontitis

Author: ChenLing
Tutor: ZuoFuHua
School: Fujian Medical
Course: Clinical Stomatology
Keywords: Oxymatrine Endotoxin Periodontitis Periodontal ligament cells Rats
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 70
Quote: 0
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Abstract


Oxymatrine (oxymatrine) on a variety of acute exudative inflammation the obvious antagonism have not been reported, but studies at home and abroad about oxymatrine in the treatment of chronic periodontitis. This study was to explore the role of prevention and treatment of oxymatrine on experimental periodontitis through animal experiments; through cytological studies of bacterial lipopolysaccharide (lipopolysaccharide, LPS) under the action of oxymatrine on periodontal ligament cells (Periodontal ligament cell PDLC) the biological behavior of oxymatrine provide experimental evidence for prevention and treatment of periodontitis. In this study, the experiment consists of two parts: the oxymatrine suppression periodontitis alveolar bone resorption in experimental research method: 25 SD rats were randomly divided into three groups: normal control group (N), blank control group (C) and the experimental group (E). Steel wire ligation rat animal models of experimental periodontitis. The experimental group was given during the modeling oxymatrine gavage, the control group were given normal saline orally. Ig six weeks after the rats were killed, periodontal histopathological changes observed under an optical microscope, measure the distance the enamel dentine sector to the alveolar crest. Results: The experimental group rat periodontal tissue inflammatory response was significantly lighter than the normal control group, the amount of alveolar bone resorption small (P lt; 0, 05). Conclusion: oxymatrine in to some extent inhibited in rats with experimental periodontitis alveolar bone absorption. 2. Oxymatrine on the biological function of bacterial lipopolysaccharide PDLC: isolation and culture of PDLC, the experimental group, respectively, for the different combinations of LPS and different concentrations of oxymatrine solution control group containing only 1 % FBS in DMEM. MTT assay was used to detect the proliferation of PDLC; cycle distribution was determined by flow cytometry PDLC; TEM observations PDLC ultrastructural changes; ALP activity of the enzyme-linked immunosorbent detected PDLC; RT-PCR detection of IL-6 and IL- 1βmRNA horizontal. Oxymatrine PDLC biological behavior by LPS. Results: LPS significantly inhibit the the PDLC growth and proliferation, DNA synthesis early cells was significantly increased DNA synthesis phase cells decreased, reducing the ALP activity of PDLC, ultrastructure injury PDLC, enhanced IL-6 and IL-1βmRNA level expression of oxymatrine improve this phenomenon. Conclusion: oxymatrine to some extent blocked LPS PDLC's destruction, inhibiting the expression of periodontitis media, has a protective effect on the PDLC.

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