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Polygonum orientale separation and analysis of flavonoids and antioxidant activity

Author: WeiYan
Tutor: ChenXiaoQing
School: Central South University
Course: Analytical Chemistry
Keywords: Polygonum orientale Flavonoids Taxifolin Extract Purification Radical scavenging
CLC: R284.1
Type: Master's thesis
Year: 2008
Downloads: 360
Quote: 5
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Abstract


This paper studies the Chinese medicine Polygonum orientale flavonoids extraction, purification and determination methods at the same time to clear 1,1 - diphenyl picrylhydrazyl (1 of Polygonum orientale flavonoids crude extract and taxifolin, 1-Diphenyl-2-picrylhydrazyl radical, the DPPH) ability to carry out a preliminary study. (1) extraction of total flavonoids. Were used to detect indicators of yield of total flavonoids, ultrasonic extraction and microwave extraction method for extraction. Through the single factor experiment and orthogonal experiment to determine the best extraction process of the two extraction methods, better microwave extraction of total flavonoids rate of 3.41%. (2) purification of total flavonoids. The compared five macroporous resin adsorption, desorption performance, select S-8 macroporous resin purification of total flavonoids of Polygonum orientale using concentration 1BV / h flow rate on the column to extract for 1.50mg/mL Polygonum orientale, 60% ethanol 1BV / h flow rate of elution the resulting total flavonoids crude purity of 83.26%. (3) Taxifolin separation. Adopt the lead salt precipitated silica gel column chromatography and preparative reversed-phase high performance liquid chromatography three methods Polygonum orientale Taxifolin separation and purification. Determine the optimum separation conditions, better preparative reversed-phase high performance liquid chromatography, and the purity of the product is up to 96.26%. (4) total flavonoids and Taxifolin, determination. Determination of total flavonoids by spectrophotometry, rutin as reference substance, too the rutin concentration C (μg / mL) and the absorbance A regression equation: A = 3.81 × 10 4 6.66 × C (r = 0.99935), rutin concentration in the linearity in the range of 8 ~ 64μg/mL a good relationship, the recovery rate of 97.5% to 103.3%; Taxifolin Determination by high performance liquid chromatography, chromatographic conditions: Lichrospher C 18 column (150mm × 4.6mm: 5μm), the mobile phase was methanol -0.3% aqueous acetic acid (35:65, v / v) isocratic elution, detection wavelength 290nm, flow rate of 0.8mL / min, column temperature of 25 ℃, was the concentration C Taxifolin peak area Y regression equation: Y = 1.54 × 10 3 1.40 × 10 4 × C (r = 0.99995), the linear range of 25 ~ 150μg/mL. (5) total flavonoids extract Taxifolin antioxidant experiments. Different solvent extraction of flavonoids crude extract clear a certain extent the ability of DPPH radical scavenging quite different, the strength of the order of methanol> ethanol> water> ethyl acetate> chloroform; clear Taxifolin. DDH free base the ability to slightly higher than the rutin confirmed Taxifolin has strong antioxidant capacity.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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