Dissertation > Excellent graduate degree dissertation topics show

The Polygona-Polysacch-Arose’s Effects upon APP Transgenic Mices’ Expression of NOS in the Brian and the Learning and Memory Ability

Author: ZhangPing
Tutor: LiYouYuan
School: Central South University
Course: Geriatrics
Keywords: polygona-polysaccharose Alzheimer’s Disease APP transgene neural Nitric Oxide Synthase inducible Nitric Oxide Synthase
CLC: R285.5
Type: Master's thesis
Year: 2007
Downloads: 109
Quote: 0
Read: Download Dissertation

Abstract


With the close relation of aging and the prolonging of the age , the morbidity of Alzheimer’s disease is greatly increasing .It is the forth cause of death in old people’s disease.The onset and progress of patient’s condition is influenced by many factors , the pathogeny is not very clear by now .Some studies suggest that it is effective way of pharmacotherapy to block the expression of iNOS of gliocyte and inhibit the activity of iNOS . Polygona-polysaccharose play an important role in pharmacological , such as anti-free-radical injure , anti-senium and anti-inflammatory and so on . Therefore, mice which had been transferred genes of APP were studied in this experiment. The aim of the present study is to investigate the effects of polygona-polysaccharose on the spatial learning and memory of APP transgenic mice, the influence of polygona-polysaccharose on biochemical indexes and hippocampus structure in APP transgenic mice, the expression of neural NOS (nNOS) and the expression of inducible NOS( iNOS )in APP transgenic mice and the influence of polygona- polysaccharose on them.The purpose is to explore the effects and functions of polygona-polysaccharose on APP transgenic mice, and try to find a new way of preventing and treating experimental AD.Objective: To investigate the effect of polygona-polysaccharose on APP transgenic mice, and to study the protective and therapeutic function of polygona-polysaccharose on APP transgenic mice.Method: Inject different concentration of polygona-polysaccharose solution into the mices’ stomach continuously for 45 days. The Morris water maze was adopted to study the ability of the mices’ initiative learning and memory; light microscope was used to observe morphology; the expression of nNOS and iNOS was detected by immunochemistry tissue staining method. Eventually, the effect of polygona-polysaccharose on the above-mentioned index was investigated.Result: The experimental result shows that polygona-polysaccharose has the following effects:1. Ethology : compared with the treat group , the delitescence of the Blank group prolonged obviously, the frequency of finding the platform decreased, and the frequency of mistake increased (p<0.01). Within the treat group, the group with high concentration polygona-polysaccharose increased in the frequency of finding the platform, shortened in the delitescence, and decreased in the frequency of mistake (p<0.05).2. Expression of nNOS and iNOS: compared with the Blank group, the energy of nNOS in the treat group was heightened(p<0.01); and the expression of iNOS was degraded (p<0.01). Within the treat group, there is significance difference. (p<0.01).3. Morphology change: the amount of neuron of the blank was less than that of the treat group. Cell Apoptosis phenomenon could be seen, for example, cell nucleus of some neuron showed pyknosis condition, caryotheca slightly thickened and aberration lipofuscin increased and so on. Polygona-polysaccharose treat group had more neurons, the changes of cell were not obvious, cell had normal structure.Conclusion: Polygona-polysaccharose can adjust the expression of nNOS and iNOS and improve the ability of learning and memory of APP transgenic mice. T. Polygona-polysaccharose is an effective prescription for the prevention and therapy of experimental AD.

Related Dissertations

  1. The Experimental Research on Using Electroacupuncture to Treat the Dysfunction Memory of Rats Suffered from Alzheimer’s Disease,R245
  2. The Effect of Simvastatin to the Appswe/PS1dE9 Transgenic Mouse with High Cholesterol Diet,R589.2
  3. Insight on Neuropsychological Characters between Different Kinds of Cognitive Impairment,R749.1
  4. Roles of Small Conductance Calcium-activated Potassium Channels in Mice with Alzheimer’s Disease,R749.16
  5. Chifeng City in parts of Alzheimer's disease and blood lipids preliminary analysis of trace elements,R749.16
  6. Serum 25 - hydroxyvitamin D levels and cognitive impairment in elderly Correlation,R749.1
  7. Ginsenoside Rg1 Protects Against β Amyloid-Induced Neurotoxicity Via Activation of Er and IGF-IR Signaling Pathways,R285.5
  8. The impact of lifestyle factors on the cognitive function of elderly,R749.1
  9. Virtual Screening for New Drug Candidates Against Alzheimer’s Disease Based on STITCH Database,R96
  10. High Level of Zinc Intake Causes AD-like Pathologic Changes and Memory Impairment,R749.16
  11. β 2 -adrenergic receptor agonist clenbuterol on PC12 cell injury and its mechanism,R96
  12. The Effects of Different Traditional Chinese Medicine Therapy on the Expression of GSK-3β in Hippocampal Region of the AD Mice Model,R285
  13. Thiazolo [3,2-a] pyrimidine Design, Synthesis and Biological Activity,R96
  14. Synthesis of Analogues Related to Physostigmine and Structure/ Activity Relationships for Anticholinesterase Activity,R914
  15. Animal Model of Alzheimer’s Disease Induced by Aβ(1-40) Combined with 192-IgG-saporin,R749.16
  16. The Study of Human Amnion Membrane Mesenchymal Stem Cell Transplantation for the Treatment of Transgenic Mice with Alzheimer’s Disease,R749.161
  17. The Mechanism of LPS Induced Injury in PC12 Cells and the Protective Effect of EPA,R742.5
  18. Mechanisms of Apoptosis of Brain Neuron of Alzheimer’s Disease-like Model Mice Induced by Excitability Nerve Toxin Kainic Acid and Protective Effect of Ferulic Acid,R965
  19. Transplantation of Bone Marrow Mesenchymal Stem Cells in Treatment of Alzheimer’s Disease in Rats,R749.16
  20. Intervention on Cartilage Cell Apoptosis by Intra-articular Injection of Hyaluronic Acid (HA) after Total Medial Meniscectomy in Rabbits,R687.3
  21. The Effects of Tonifying Kidney and Supplementing Qi and Promoting Blood Recipe and the BMSCs on COL II and iNOS of Cartilage and IL-1β of Serum in Rabbits with Knee Osteoarthritis,R285.5

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile