Dissertation > Excellent graduate degree dissertation topics show

Selective Detection of Viable Pathogens in Water Using Propidium Monoazide Combined with qPCR and Its Application

Author: ZuoTieZuo
Tutor: HeMiao
School: Tsinghua University
Course: Environmental Science and Engineering
Keywords: PMA-qPCR Active bacteria Pathogen detection Sewage treatment Disinfection characteristics
CLC: R123.1
Type: Master's thesis
Year: 2010
Downloads: 113
Quote: 0
Read: Download Dissertation

Abstract


Rapid and accurate detection of pathogens in water activity on the prevention of water-borne disease outbreaks and protect water quality and safety is important. In this study, the conventional techniques can not distinguish between active bacteria qPCR and the lack of non-active bacteria, the nucleic acid dye established a PMA (propidium monoazide) combined with qPCR detection method (PMA-qPCR), the full activity of the membrane can be pathogens selective detection, studies using this method to analyze typical pathogen indicator bacteria in the sewage treatment process to remove features, especially for common disinfection disinfection properties of E. coli do in depth. The main conclusions are: (1) PMA dye in qPCR analysis to effectively remove bacteria from non-active DNA, and the DNA not active bacteria significant impact on the detection result, PMA-qPCR method can complete the sample cell activity for selective detection of bacteria, but the method for different types of active bacteria selective detection results vary. (2) PMA-qPCR method is applicable to sewage treatment plant influent, secondary effluent, secondary clarifier sludge activity in real samples of different selective detection of bacteria, but the primary sludge will significantly inhibited PMA-qPCR choice detection results, the application of this method has limitations. (3) PMA-qPCR method of sewage treatment process pathogen indicator bacteria culture test results and test results had a good linear correlation, and the difference was not significant, compared with the conventional qPCR, the method of detection results more close to the culture, indicating that PMA-qPCR method can meet the sewage treatment process active pathogen testing requirements. (4) PMA-qPCR, qPCR and conventional culture method test results together indicate that a treatment process for E. coli and Enterococcus faecalis were no significant removal, secondary treatment process of these two pathogenic indicator bacteria removal In 1.5 log10 or so. (5) PMA-qPCR method to characterize two kinds of chlorine and chloramine disinfection an inactivation of E. coli. The method of chlorine and monochloramine inactivation curves of E. coli meet a kinetic equation, inactivation rate constants were 2.24 L / (mg · min) and 0.0175 L / (mg · min), lower than that for France inactivation rate constants obtained, indicating that the pathogen chlorine and chloramines a rate lower than its destruction of cell membranes may develop resistance to the pathogen destruction rate. However, current research indicates that PMA-qPCR is still unable to accurately assess the UV disinfection for the inactivation of E. coli.

Related Dissertations

  1. Sewage treatment equipment design and application of automated operation,TP273
  2. Port industrial zone planning and layout of the sewage treatment system,X703
  3. Control System Design of the Sewage Treatment Plant Project in Hainan Province Qinglan,X703
  4. One kind of large volume water discharge characteristics of the corona discharge reactor research,TM832
  5. Shijiazhuang Economic and Technological Development Zone A sewage treatment plant a transformation process of the mentioned standard,X703
  6. A Study on Constructed Wetland Restoration of Shen-Fu Irrigation Channel’s Sewage,X703
  7. The Experimental Research on CEPT+A/O Purification Method of Municipal Sewage in the Rivers Which Inflood into DianChi,X703
  8. Study on the Key Problems of Flat-sheet Membrane Bioreactor for Engineering Application,X703.3
  9. Research on Technology of Ferric Enzymatic Activated Sludge,X703.1
  10. Mianyang pond flood sewage treatment plant automation systems research and development,X703
  11. Comparison and Study of the Various Dominant Microorganisms Treatment of Different Municipal Wastewater Treatment,X703
  12. The Research on Controlling and Soft Seneor in Treating Sewage,X703
  13. Computerized Quantitative Method Comparision and Selection of Municipal Wastewater Treatment Techniques and Application,X703
  14. Chen Bridge Zhengzhou Sewage Treatment and Reuse Project Evaluation,X505
  15. Research on Investme Decisions of Sewage Treatment Based on Multi-Objective Plannng Model,F224;X52
  16. Distribution Characteristics and Volatilization Rules of Volatile Organic Chemicals from WWTP,X703
  17. The Research of Two-stage BAF in Water Treatment in the Residential Areas,X703
  18. Rural sewage and garbage treatment technology research,X703
  19. Research on Intelligent Optimal Control Method for Wastewater Treatment Process,X703
  20. The Sewage Treatment Plant Energy Consumption Analysis and Energy Conservation Technology Research in the City of Kunming,X703

CLC: > Medicine, health > Preventive Medicine,Health > Environmental health,environmental medicine > Water and water supply sanitation > Health surveillance of the health standards of water and water
© 2012 www.DissertationTopic.Net  Mobile