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Using genetic differences AFLP and SRAP two molecular markers 4 shiitake strains and four strains ( two of which belong to the oyster mushroom Pleurotus one belonging Bailing , the other one belongs to citrinopileatus ) . The results showed that the AFLP markers , four mushrooms strains between the two ostreatus strains , three Pleurotus genetic similarity coefficients between different species and Lentinus and Pleurotus were 0.74 ~ 0.89,0.89,0.47 to 0.89 and 0.44 , mushrooms isolates revealed by SRAP markers ostreatus strains Pleurotus genetic similarity coefficients between three different kinds of Lentinus and Pleurotus 0.70 to 0.93,0.93,0.47 to 0.93 and 0.53 . Pursuant to the preliminary view that the same kind of mushroom genetic similarity between different strains coefficient of about 0.70 or more , Pleurotus mushroom, Bailing , elm Huangmo three kinds of genetic similarity coefficient of about 0.5 to 0.7 , Lentinus and Pleurotus genetic similarity coefficient between the two genera are generally no higher than 0.5. Two molecular markers analysis results also showed that two oyster mushroom strains individual full-sib and half-sib individuals showed \The genetic similarity coefficient is less than full-sib strains . Half-sib strains somatic incompatibility and the intensity of the reaction is greater than the full-sib strains , molecular marker analysis results coincide . The two mushrooms strains of full-sib and half-sib strains cluster analysis showed that using SRAP markers tested strains showed each of the more obvious of full-sib and half-sib priority clustered into a tendency , compared using AFLP markers , this clustering tendency is weaker , however , belong to two groups of strains are mostly able to clearly distinguish , in a comprehensive analysis of the two markers be considered . In summary, the application of these two molecular markers to identify mushrooms Pleurotus strains is feasible , and with increasing genetic distance between the strains , the genetic similarity coefficient is reduced accordingly .
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