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Color Doppler ultrasound irradiation on expression in fetal rat central neuronal apoptosis and Caspase3, Caspase8

Author: LiXiuQin
Tutor: JiChunZuo
School: Shanxi Medical
Course: Pathology
Keywords: Ultrasound Neurons Apoptosis Caspase3mRNA Caspase8mRNA
CLC: R714.5
Type: Master's thesis
Year: 2008
Downloads: 23
Quote: 0
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Abstract


Objective: To investigate the diagnosis of possible mechanisms of central neuronal apoptosis and apoptosis by color Doppler ultrasound, and clinical application of ultrasound in obstetrics security provided new evidence. Methods: 20 pregnant 12-14d adult Kunming mice as in pregnant model by ultrasonic irradiation time were randomly divided into four groups, group I (control group), Ⅱ group (irradiation group 10min), Ⅲ group ( irradiation 20min group), group Ⅳ (irradiation 30min group). Instruments used the Yum DU8 color ultrasound diagnostic apparatus, irradiation conditions: abdominal convex array probe, black and white frequency of 3.0MHz, the color frequency of 2.0MHz, MI = 0.7, TIS = 0.6, TIB = 1.7, 24 h after irradiation take fetal rat brain specimens. A conventional producer, in situ detection of apoptosis (TUNEL) detection of apoptotic cells, each slice select 20 high-power field, normal cells and apoptotic cells per HPF count of 500, calculated apoptotic cells in the average percentage of the total number of cells, the apoptosis index (apoptosis index AI). 2. Fetal central neurons in Caspase3mRNA, Caspase8mRNA expression by RT-PCR analysis. The data obtained were statistically analyzed using SPSS11.5. Results: 1. Apoptosis-positive cells in the nucleus dyed a darker tan or light brown-yellow, negative nuclei were blue. Control group and irradiation the 10min group of fetal rat central neurons can be seen scattered apoptotic cells, apoptotic index irradiation 10min group and control group difference was not statistically significant (P gt; 0.05); irradiation 20min group, irradiation 30min apoptotic cells easier observed, the individual specimens part of the area visible more intensive apoptotic cells. Irradiation 20min central neuronal apoptosis index with the control group, irradiation the 10min group difference was statistically significant (P lt; 0.05), ultrasonic irradiation central neuronal apoptosis increased more than 20min; irradiation time to 30min apoptotic cells continued to increase, the apoptotic index with the control group, 10min 20min group comparison difference was statistically significant (P lt; 0.05). 2.Caspase3, Caspase8 in each group were expressed using Bioimagining System imaging analysis system to observe the results of electrophoresis, electrophoretic band of the amplification product to be scanned and for semi-quantitative detection. Caspase3, Caspase8 expression in irradiated 10min group compared with control group difference was not statistically significant (P gt; 0.05); the irradiance 20min group, irradiation 30min group activation of Caspase3, Caspase8 and increased expression of statistically significant difference compared with the control group (P lt; 0.05); irradiation 10min, 20min group and 30min group activation of Caspase3 Caspase8 and expression in turn increase their respective pairwise comparison difference had statistical significance (P lt; 0.05). Conclusion: biological effects from the recent diagnosis with color Doppler ultrasound after abdominal the continuing irradiation in pregnant mice the uterus projection area of ??more than 20min can cause an increase in fetal rat central nervous system neuronal apoptosis; simultaneously Caspase3mRNA, Caspase8mRNA increased expression, suggesting that membrane pathway may be central neuronal apoptosis caused by one of the signal transduction pathway. Diagnostic color Doppler ultrasound long irradiation can cause embryonic central nervous potential injury to avoid long irradiation on embryonic sentinel.

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CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Fetus
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