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Thepreparation of VEGF/bFGF-impregnated Microsphere and Its Effect on the Survival of Ischemic Dorsal Random Skin Flap in a Rat Mode

Author: WangZuo
Tutor: GuoShuZhong；MaXianJie
School: Fourth Military Medical University
Course: Surgery
Keywords: Vascular endothelial growth factor Basic fibroblast growth factor Microspheres Sustained-release Random flap Survival
CLC: R622
Type: Master's thesis
Year: 2008
Downloads: 82
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Abstract


Objective: Preparation of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and release microspheres of biodegradable gelatin examine the preservation of its biological activity and the role of endothelial cells (EC); its role in the dorsal random flap examine different growth factor sustained release and proportional growth factor together on flap survival. Method: preparation emulsified condensation method using a modified cross-linked composite of bFGF, VEGF gelatin release microspheres under the microscope to measure the apparent traits, encapsulation efficiency, drug loading efficiency and dynamic dialysis method to measure the release characteristics; them Jiaruneipi in the cell culture medium and injected in the dorsal random flap cell count, four MTT microreaction colorimetry (MTT) Determination of endothelial cell proliferation and vitality; flap survival, angiogenesis the count and flap blood flow detection. Results: Gelatin release microspheres average particle size of 11, 32 ± 3. 64μm; the of bFGF average drug loading efficiency and encapsulation efficiency was 0.26%, 90.6%, of VEGF average drug loading and encapsulation efficiency was 0.22%, 88.4 %. 7 days after the measuring cumulative release rate of 93.1% and 90.6%, respectively. Its role in cultured endothelial cells, bFGF and VEGF release microspheres cell count of 24.0 ± 2.16,29.16 ± 3.65, the absorbance (A) 18.74 ± 2.21, 0.756 ± 0.042,1.023 ± 0.106, and the control group cell count absorbance (A) 0.651 ± 0.064 The difference was statistically significant (P lt; 0.05). The two factors combined cell count was 34.51 ± 4.79, absorbance (A), 1.328 ± 0.098, significantly higher growth factor alone (P lt; 0.05). Injected in the dorsal random flap 7 days after the flap survival rate was 45.43 ± 2.71%, respectively; 58.45 ± 2.70%; 68.54 ± 2.79%; 54.38 ± 4.52%; 65.42 ± 2.19%; 63.75 ± 2.24 %; 78.33 ± 3.23%, the release agent group survival rate was significantly higher than that of the control group and the the purely growth factor group (P lt; 0.05), combination of two factors flap survival best quality; flap capillary density respectively. 18.68 ± 5.44; 25.41 ± 4.06; 31.16 ± 4.38; 21.28 ± 3.82; 28.75 ± 2.36; 32.74 ± 4.67; 39.32 ± 3.73, compared with the control group with a significant difference (P lt; 0.05). Two kinds do not have the scale factor combined flap survival and capillaries were VEGF / bFGF = 1:1 group: 63.75 ± 2.24%, 32.74 ± 4.67; VEGF / bFGF = 1:2 group 65.79 ± 3.48%, 35.67 ± 3.25; VEGF / bFGF = of 1:3 group 77.5 ± 3.95%, 40.38 ± 4.19. Conclusion: 1. Improved emulsified condensate release microspheres prepared Gelatin is a simple, effective way of processing. The preparation process is simple, grain and uniform diameter of the microspheres, the high rate of drug-loaded with a good slow-release activity, carrier gelatin tissues toxic side effects. 2. Gelatin microspheres can significantly extend the half-life of the growth factor to promote the process of vascular endothelial cell proliferation and any flap to promote the survival of the flap, controlled release drug prolongs the time of drug action, and a good way to reduce the dosage. 3.bFGF and VEGF have synergistic effects, used in combination can significantly speed up its vascularization promotion of ischemic skin flap survival and reduce the dose, the dose is strong synergies arising 3:1.

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CLC: > Medicine, health > Surgery > Plastic Surgery (repair surgery ) > Plastic surgery school
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