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Study of Time-Resolved Laser Induced Fluorescence Immunoassay

Author: ZhangHe
Tutor: LuJun；PanLiHua
School: Changchun University of Science and Technology
Course: Optics
Keywords: laser-induced fluorescence time-resolved RE fluorescence chelates
CLC: R446.6
Type: Master's thesis
Year: 2008
Downloads: 118
Quote: 0
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Abstract


Time-resolved fluorescence immunoassay technology is a new generational non -radioactivity immunoassay.It has wide application and bright foreground in biotechnology and clinical detection.In order to overcome the shortage of liquid-phase TRFIA system,a solid-phase TRFIA system was raised in thesis,UV laser is the excited resource,at the same time,BOXCAR complete time-resolved.Photodiode diode and photomultiplier tube as synchronously triggering and receiving wares.A concrete method to actualize focusing,filtering and prismatic optical system was developed.The integral programme of time-resolved laser induced fluorescence immunoassay system was designed.According to the programme,the detection device was established.Four kinds of RE（rare-earth） five phosphate crystals and Eu（β-NTA）3（TOPO）2 rare earth fluorescence chelates were detected time-resolved spectrum and fluorescence lifetime by this detection device,the detection limit is Eu3+10-12mol/L,at last gives the relation curve of fluorescence intensity and the fluorescence concentration.

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CLC: > Medicine, health > Clinical > Diagnostics > Laboratory diagnosis > Immunology Examination
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