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The purpose of the PCR method , the Tannerella forsythia bacteria measured in elderly Uighur (Tannerella forsythensis, Tf) detection rate , and to explore the consistency of saliva samples and subgingival plaque samples tested in patients with chronic periodontitis . Methods Hotan Prefecture Uygur elderly 96 cases , of which 71 cases of chronic periodontitis patients , 25 cases of edentulous . Collecting saliva and subgingival plaque samples with a concentration of 25% Chelex- 100 ( a chelating resin ) , and 56 ° C were incubated for 30 min , 100 ° C to boil for 10 min , after centrifugation , the DNA template was prepared . Design Tf 16S rRNA primers , respectively, saliva and subgingival plaque samples for PCR amplification , amplification product was cloned nucleotide sequence was determined and concept the bacteria detection察福赛斯坦纳rate , calculate the Kappa value . Result of the Tannerella forsythia bacteria in the stationary saliva and subgingival plaque samples detected with a high degree of consistency , the detection rate was 76.06% ( 54 /71) and 90.14% ( 64 / 71 ) , Kappa value of 0.322 , consistent rate of 80.28% . Tf of the 16S rRNA gene fragment cloning and sequencing results Blast. Genebank registered 16S rRNA gene fragment homology to 100%. Conclusions (1 ) the present study found a higher detection rate in the Hotan Prefecture Uygur elderly patients with chronic periodontitis Tannerella forsythia bacteria . ( 2 ) The primers used in the test can be used to Tannerella forsythia detection of bacteria in the oral cavity , especially saliva samples used in the experiment , and easily obtained, it is possible instead of subgingival plaque samples .
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