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Objective: To simulate the human posterior discectomy using lateral passing the fibrous ring puncture aspiration nucleus pulposus establish rabbit disc degeneration animal models to analyze the feasibility and advantages, and imaging and pathology detection analysis reproduction of the objective law of development of disc degeneration process, more ideal animal model for the study of intervertebral disc degeneration, and to provide an experimental basis for the application of tissue engineering methods such as repair and reconstruction of intervertebral disc. Methods: 20 Japanese white rabbits, each rabbit L1 / 2 and L3 / 4 disc included in the experiment group, L2 / 3 intervertebral disc included in the control group. New line Sumianxin preoperative intramuscular injection of anesthesia, routine preoperative preparation, take the left lateral decubitus position, right posterior lateral approach along the rabbit L1 / 2 and L3 / 4 right posterolateral intermuscular septum separating exposed transverse process and down \side parallel to the direction of the endplate piercing depth control in 5mm, then the syringe Withdrawing about 10ml, suck out the 5.0-8.0mg nucleus pulposus. Incision sutured Close. Regained consciousness after animals were randomly divided into 2, 4, 8 and 12 weeks, four model group, n = 5 conventional breeding cage. Postoperative be three days of intramuscular gentamicin to prevent wound infection. Preoperative and postoperative 2, 4, 8, and 12 weeks after the experimental and control groups, respectively disc line CR and MRI, respectively, the rabbits were sacrificed take L1 / 2, L3 / 4 and L2 / 3 intervertebral disc Masson staining Observe the pathological changes of the intervertebral disc tissue. Results: (1) imaging analysis showed that 2 weeks after the first 12 weeks, X-ray visible experimental group intervertebral space narrowing, DHI% with modeling time extension is reduced, compared with the control group, the differences of each time period were significant (P <0.05), the cartilage endplate and the vertebral edge increased bone density. MRI shows the experimental group, the nucleus pulposus area narrow, the annulus fibrosus area increases reduce intradiscal MRI T2 weighted signal. Nucleus pulposus signal strength was gradually decreased, compared with the control group stage MRI improved the Thompson grading gradually tend serious classification, the results of the difference was statistically significant (P <0.05). (2) pathology tests showed the Masson staining the nucleus pulposus of the control group in the nucleus pulposus morphology rules, clear the fibrous ring boundaries, the nucleus pulposus cells more the fibrous ring neatly dense; experimental group the nucleus pulposus fractured formation, collagen arrangement disorder , reduce the number of nucleus pulposus cells; grow with time, the cartilage-like cells reduce the number the nucleus pulposus gradually fibrosis, the annulus fibrosus gradually layered disorders, twisted, broken. Each time period, compared with the control group Nishimura intervertebral disc pathological grade differences were significant (P <0.05). Conclusions: (1) the experimental analysis of imaging and histopathology confirmed the basic characteristics of rabbit intervertebral disc degeneration; (2) Analog posterior discectomy, carried out after the outer fibrous ring aspiration of nucleus pulposus can induced rabbit intervertebral disc degeneration slowly, can be reproduced disc degeneration lesion development objective laws, and model-making simple, reproducible, and is an ideal animal model to study the etiology and pathophysiology of disc degeneration, but also for the study of clinical disc herniation recurrence and validate reliable, and effective treatment of disc degeneration, such as growth factor therapy, gene therapy, cell therapy, tissue engineering to provide an experimental basis.
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