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Studies on the Isolation from Cauliflower Seeds and Anti-Allergic Activity of Glucosinolates

Author: CuiHongGang
Tutor: DuZuoZhen
School: Zhejiang Technology and Business University
Course: Of Food Science
Keywords: Glucosinolates Countercurrent chromatography Zealand Seed Separate Antiallergic activity
CLC: R284
Type: Master's thesis
Year: 2008
Downloads: 163
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Abstract


Countercurrent chromatography is a technology of liquid-liquid partition chromatography without solid support or carrier , it can achieve continuous and efficient separation and preparation . Glucosinolates (referred ehioglucoside ) is widely found in cruciferous plants sulfur secondary metabolite . Such compounds and their degradation products having a wide range of biological activity , can be suppressed not only result in the growth of bacteria and microorganisms , and also has a good anticancer activity . Therefore, the separation and purification of glucosinolate its structure identification in food science , biomedical important practical significance . This thesis applies countercurrent chromatography separation Zealand Seed glucosinolates , lay a technical foundation for the separation and preparation of glucosinolates . Countercurrent chromatography separation Zealand Seed glucosinolates solvent system , to find a suitable solvent system n-butanol - acetonitrile -10 % ammonium sulfate solution ( 1:0.5:2.2 ) . Column volume of 1200 ml the HSCCC-D1200 high-speed countercurrent chromatography separation of crude extract , and five kinds of glucosinolates monomer was further purified by preparative high performance liquid chromatography : (Ⅰ) the ene -butyl glucosinolate ( II ) 4 - pentenyl thioglucoside glycoside ( III ) 3 - methylethylene sulfonyl n-propyl thio - glucoside (IV ) 4 - methylethylene sulfonyl n-butyl thioglucoside glycoside ( V ) 7 - methyl n-heptyl group , a sulfinyl group glucosinolates . Their structures were identified by ESI-MS and 1HNMR, 13CNMR . Adding 1mg of Compound I - in 1ml of hyaluronidase ( 346U./ml ) solution VII ( I ) the alkylene butylthiapentalene glycoside ( II ) 4 - pentenyl thioglucoside glycoside ( III ) 3 - methylethylene sulfonyl group n-propyl thioglucoside the glycosidic ( IV ) 4 - methylethylene sulfonyl - n-butyl- thio - glucoside (Ⅴ ) 7 - methylsulfinyl - n-heptyl thioglucoside ( Ⅵ ) allyl glucosinolates , (VII) 4 - methylsulfinyl -3 butenyl glucosinolates measuring their hyaluronidase inhibition test results showed that : Ⅳ GT ; VII GT ; II GT ; the Ⅴ GT ; Ⅲ gt; Ⅰ gt; Ⅵ. High concentrations of anti-allergic activity and in vivo anti- allergic activity test needs further study.

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