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Pueraria the (Raix Puerariae) is a kind of traditional Chinese medicine, contains a variety of active ingredients, which puerarin, daidzein, and soy aglycone isoflavones. Can be used for the treatment of cardiovascular disease, angina and arrhythmia disease puerarin, daidzin and daidzein can be used for the treatment and prevention of disease in women. Analysis and purification of isoflavones puerarin reduced Pueraria own use value. In addition, the research and development of Pueraria lobata (Puerariae Lobata (wild) Ohwi) are concentrated in Gehua parts Pueraria, and kudzu site analysis for isoflavones, analysis and biological activity of the other active ingredients research. Maoshan region Pueraria lobata lobata resources for further research and development the Maoshan areas of study, research analysis of isoflavones, chemical composition and antioxidant activity of Separation and Purification and kudzu, Pueraria product quality provide a basis for the establishment of standards and Maoshan kudzu comprehensive development and utilization. Pair the Maoshan of kudzu in puerarin, daidzin and daidzein content while determination studies have not been reported Pharmacopoeia 2005 edition Gegen determination of puerarin pretreatment apply to Pueraria puerarin, daidzin and daidzein, while determination to be studied. Therefore, this paper studies the simultaneous determination kudzu puerarin, daidzin and daidzein pre-treatment and reversed-phase high performance liquid chromatography (RP-HPLC) analysis method. Sample pretreatment ultrasonic extraction with 70% ethanol, 50 ° C 20min, RP-HPLC directly measured, and compared with the pharmacopoeia method. Reference substance puerarin, daidzin and daidzein showed a good linear relationship in the range of 0.2140 ~ 1.2840,0.0178 to 0.1066 and 0.0044 ~ 0.0026μg, of the correlation coefficient r 0.9998,0.9997 and 0.9994, respectively, the average recoveries were 99.46 %, 104.29% and 111.54%, RSD were 2.20%, 4.60% and 6.60% (n = 5). Precision, stability and good reproducibility. Pueraria measured in ultrasonic extraction puerarin, daidzin and daidzein content were 3.98%, 0.36% and 0.039%, higher than the the Pharmacopoeia extraction measured these three compounds content, and more suitable for the simultaneous extraction of puerarin daidzin and daidzein. Ge stem of puerarin, daidzin and daidzein content of 40mg/kg, 32mg/kg and 6.6mg/kg, Ge leaves puerarin, daidzin and daidzein content of 16mg/kg, 50mg/kg and 12mg/kg. The method is accurate, fast, and simple operation, good separation, can be used Pueraria lobata root, stems and leaves of puerarin, daidzin and daidzein content Simultaneous determination; the Maoshan area kudzu stems and leaves puerarin daidzin and daidzein content is significantly lower than the the Pueraria content. The many the macroporous reported on isoflavones, but are mainly puerarin purified. This paper focuses on a can also be obtained from Pueraria variety of active ingredients puerarin, daidzin and daidzein macroporous resin purification method. Through static adsorption, static the desorption experiment investigated AB-8, D-101, NKA and D1300, four macroporous adsorption resin AB-8 resin resin Pueraria lobata flavone amount of adsorption rate and resolution, preferably, and examine and Optimizing AB-8 Pueraria lobata flavone separation and purification process conditions. The results showed that AB-8 resin adsorption effects of Pueraria lobata flavone adsorption capacity of 116.33 mg / g; Langmuir equation described the adsorption isotherm; AB-8 Resin Separation and Purification of Pueraria Total Flavonoids appropriate conditions injection mass concentration of 9.63g / L, a flow rate of 1.5 mL / min, and eluted with distilled polysaccharide impurities, using 10%, 30%, 50%, 70% ethanol successively gradient elution, segments eluate Save pressure concentrated and freeze-dried to obtain the purified product of the different active ingredient. 10% ethanol can be obtained as the main active ingredient purified puerarin content of 87.17%; 30% ethanol can be obtained as the main active ingredient purified puerarin and daidzein, puerarin and daidzein content were 53.72% and 18.41%; 50% ethanol and 70% ethanol eluent main daidzein, daidzein content of 72.41% and 74.31%, respectively. Gradient elution with the different active ingredients can be obtained purified isoflavones. Maoshan region kudzu isoflavones puerarin, daidzin and daidzein content is very low, for further study of whether they contain other active ingredients in the kudzu, a preliminary study Maoshan wild kudzu extract parts. - Ether extraction, ethanol extraction and water extraction and alcohol precipitation wild kudzu extract, volatile oil, ethanol extract and polysaccharides three different extraction site, and preliminary analysis of its components by steam distillation. Sulfuric acid the catalytic methyl ester of derivative kudzu volatile oil gas mass spectrometry (GC-MS) analysis, the results show that 70 153 components kudzu isolated components can be identified, and the alcohol compound (9) accounted 7.44% of the total; ketones (2) accounted for 4.41%; olefin compound (3) 0.65%; alkanes (5) 0.76%; aldehydes (7) accounted for 0.43%; alkyl acids (22% of 53.23%; acid compounds (11) accounted for 18.45%; aromatic compounds (4) 0.65%; esters (4) 3.55%; phenolic compounds (1 a) 0.25%; other compounds (1) accounted for 1.35%. spectrophotometry of total flavonoids of wild kudzu alcohol extract measured results show that the UV detection method rutin as reference substance measured total flavonoid content was 5.66% and 1.95%, the measured UV detection method as reference puerarin total flavonoids content of water extraction and alcohol the crude polysaccharide content was determined by Shen obtained, kudzu crude polysaccharide yield was 8.39% , the polysaccharides in the polysaccharide content of 30.31%, to visible the Maoshan region kudzu contain abundant polysaccharides. DPPH method, o-phenanthroline method and pyrogallol phenol autoxidation studied kudzu volatile oil, ethanol extract and the crude polysaccharide radical scavenging activity. kudzu alcohol extract, crude polysaccharide and volatile oil have free radical scavenging activity and scavenging effects are the most crude polysaccharide, followed by alcohol extract antioxidant activity of the volatile oil the weak for DPPH ·, volatile oil, ethanol extract and crude polysaccharide IC 50 values ??were 3.3011,1.0767 and 0.1229mg/mL (the Vc IC 50 value of 0.0091 mg / mL); · OH, volatile oil, ethanol extract and crude polysaccharide IC 50 the values ??were 3.0755,1.5615 and 0.7511mg/mL the (Vc IC 50 value 0.3477mg/mL).; the O 2 - , volatile oil, alcohol extract and crude polysaccharide IC 50 values ??were 2.3424 1.1460 and 0.9229mg/mL (Vc of the IC of 50 value of 0.2299 mg / mL). visible, kudzu crude polysaccharide and alcohol extract has good antioxidant activity, antioxidant components pending further study, Maoshan region kudzu-depth research value.
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