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Effects of Over-training on the Morphosis of the Intestinal Mucosa in Rats and Intervention of Glutamine or Soy Protein Isolate Supplementation

Author: GuMing
Tutor: JinQiGuan
School: Yangzhou University
Course: Human Movement Science
Keywords: Overtraining Small intestinal Endotoxin Ultrastructure Free radical metabolism Glutamine Soybean peptides
CLC: G80.2
Type: Master's thesis
Year: 2008
Downloads: 102
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Abstract

Experimental Objective: In this study, rats exhaustive exercise training overtraining model to explore Overtraining on Intestinal mucosal morphology and its mechanism, and explore the glutamine supplement or soybean peptide overtraining large intervention of rat intestinal mucosal morphology and its mechanism. Experimental methods: selection of 41 healthy male SD rats were randomly divided into control group (C group, n = 11), over-training group (HT group, n = 10), the the Gln intervention group (HT Gln; n = 10) and the the SPI intervention group (HT SPI, n = 10) group. Overtraining rats exhaustive exercise training, exercise six days a week, for nine weeks. The Gln intervention group and SPI in the intervention group training overtraining group average, and before each training session, subjects were supplemented with glutamine (dose of 1.5g.kg-1 body weight) or soybean peptide (dose of 0.5g.kg -1 body weight). After 9 weeks, detected in rat plasma endotoxin, HB, serum T, C, T / C, the content and the intestinal tissue Gln, MDA content, SOD activity, and to observe the morphology and ultrastructure of the small intestine. Experimental Results: ① Compared with control group, over-training group rat serum testosterone concentration ratio T / C significantly reduced serum cortex ketone concentration was significantly elevated blood Hb, WBC number was significantly reduced; supplement Gln significantly increased overtraining large rat Hb, serum testosterone concentrations, T / C ratio, significant reduction in serum corticosterone concentration; supplement SPI significantly increased overtraining rat Hb, serum testosterone concentrations, but there was no significant effect on serum corticosterone and T / C. ② Compared with the control group, the HT plasma endotoxin content was significantly higher (P <0.01); although the the SPI intervention group plasma endotoxin concentration was significantly higher than the Gln intervention group (P <0.01), but the the Gln intervention group and SPI intervention group plasma endotoxin levels were significantly lower than the HT group (P <0.01). ③ Compared with the control group, the the HT rats villi derangement, fluff the average height of a downward trend, but the difference was not significant, the fluff number decreased significantly (p <0.01); compared with the HT group, Gln group still arranged neatly, and SPI group villi height and increase in the number, but there was no significant difference. ④ electron microscopy, close control rats epithelial absorption cell surface microvilli, rules and tidy; rich in the cytoplasm mitochondria and rough endoplasmic reticulum, normal morphology; epithelial cells connected properly. HT group rat intestinal epithelial cells microvilli extremely disorders, mitochondrial degeneration, the formation of myelin-like structure; rough endoplasmic reticulum normal morphology; wider gap between the epithelial cells; disorders at the same time micro- The villi see inflammatory cell infiltration. Gln intervention group rat intestinal epithelial microvilli basic neatly arranged close; significant increase in the number of cytoplasm rough endoplasmic reticulum, contains a lot of vesicle-like substances in the vicinity; rich in mitochondria, normal structure, scattered around the nucleus; epithelial cell gap junction close morphologically normal. SPI intervention the rats intestinal epithelial cells Microvilli basic rules, rich in the cytoplasm mitochondria and rough endoplasmic reticulum, normal form, found in cells near the capillary is very rich in the endoplasmic reticulum. The close connection between the epithelial cells found in some cytoplasmic vesicle-like substances increased. ⑤ Compared with group C, the HT group and the SPI intervention group intestinal tissue Gln content were significantly reduced; the Gln group intestinal tissue was significantly increased (P <0.01) compared with the HT group, Gln intervention, the SPI intervention group intestinal tissue Gln content is further reduced, but there was no significant difference. ⑥ and group C, the HT group small intestine tissue MDA content significantly increased, SOD activity was significantly decreased (P <0.01); Compared to the HT group, Gln intervention group intestinal tissue MDA content was significantly decreased (P <0.05), SOD Although the activity has increased, but no significant difference; the SPI intervention group intestinal tissue MDA was significantly decreased (P <0.05), SOD activity was significantly higher (P <0.01). Conclusion: (1) over-training model established in this study in rats is successful. In the process of sports training supplement the Gln or complement SPI can be effective in preventing the occurrence of overtraining. (2) over-training can increase the permeability of the rat small intestine, and the process of sports training supplement Gln or SPI has an important role to maintain the permeability of the training the rat small intestine. (3) excessive training can make the rat small intestine atrophy, intestinal epithelial cell injury, and the process of sports training supplement the Gln or SPI to some extent effective in preventing training rat small intestine atrophy and intestinal epithelial cell damage. (4) excessive training Gln and a decrease of free radicals in enhanced intestinal tissue, may lead to overtraining the rat small intestine atrophy, pathological mechanisms of intestinal epithelial cell damage and increased small intestinal permeability. (5) In the process of sports training supplement Gln may maintain training rat intestinal tissue Gln levels and enhanced the antioxidant capacity of the small intestine to maintain the morphological structure of the training the rat small intestine, SPI supplementation may primarily by enhancing the small intestine The antioxidant capacity of the organization to maintain the morphological structure of the training the rat small intestine.

Effects of Over-training on the Morphosis of the Intestinal Mucosa in Rats and Intervention of Glutamine or Soy Protein Isolate Supplementation

Abstract

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