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Safflower meal Feruloyl 5 - hydroxytryptamine (FS) and coumaric acid 5 - serotonin (CS) has antioxidant, anti-tumor and anti-inflammatory antimicrobial effect, can be used in food, medicine, cosmetics and other fields, has broad development prospects. This paper systematically studied safflower meal ferulic acid 5 - serotonin and coumaric acid 5 - serotonin enrichment processes. Through single factor experiment and orthogonal experiments to determine benzene acryloyl 5 - serotonin compound extraction process as follows: 60% ethanol extraction solvent, extraction time 90min, extraction temperature 65 ℃, solid-liquid ratio 1:20, 35 mesh material pulverizing, sieving; Benzene acryloyl 5 - serotonin compound yield of 0.784%, crude extract yield was 9.96%, crude extracts benzene acryloyl 5 - serotonin compound purity of 7.87%, ferulic acid 5 - hydroxytryptamine (FS), Coumadin acid 5 - serotonin (CS), coumaroyl serotonin single glucoside (CSG), ferulic acid glucoside single serotonin (FSG) contents were 3.82,2.58,0.53 and 0.94%. Of 60% ethanol was extracted safflower meal benzyl acryloyl 5 - HT compound kinetics were analyzed to Fick's first law is based has been extracted shrinking core model to determine the dissolved benzene acryloyl 5 - HT compounds for red Seed meal particle channel for the control of the particle surface diffusion rate step, and the extraction time t and a linear relationship, the results show that the model is suitable for prediction, design and optimization. Using β-glucosidase crude extract benzene acryloyl 5 - serotonin glycoside hydrolysis by single factor test and response surface optimization of enzymatic analysis conditions were: crude extract concentration of 3.3g / L, pH = 5.9,47 ℃, enzyme dosage 3.00% (g / g crude extract), hydrolysis 10.5h, FSG and the CSG conversion rates were 94.6% and 90.0%, in the FS and CS transformant content 3.46% and 4.30, respectively, %, the enhancement of the contents of the FS and CS. Using D818 macroporous weakly basic anion exchange resin treatment of the enzyme solution FS, CS were enriched, optimum adsorption and desorption conditions: Injection solution FS, CS concentrations were 30-50,25-41 μg / mL, pH = 9.1, absorption flow 1.5mL/min; pH3.2 as eluent 80% ethanol elution flow rate 1.0mL/min, FS, CS, respectively, 86.3% yield and 85.6%, FS, CS contents were reached 23.6% and 19.4%, reaching enrichment purposes. Using XDA-1 macroporous resin desorption ion exchange column was purified to obtain crude mixture FS and CS, where FS and CS contents were 32.2% and 25.9%, yield 85.4% and 83.3%. Using silica gel column chromatography, petroleum ether / acetone gradient system, empty tower velocity 0.074cm/min, injection volume 0.012g / g silica gel, get FS and CS purity of 49.3% and 40.2%. Mixture yields were 91.3% and 92.7%. HPLC-ELSD, infrared spectroscopy and mass spectrometry showed that enrichment and purification process does not alter the structure of FS and CS.
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