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The Relationship between Calcineurin and Myocardium Apoptosis and Effects of Carvedilol Intervention

Author: YinWenJie
Tutor: ShenXiaoMei
School: Shanxi Medical
Course: Geriatrics
Keywords: Calcineurin Carvedilol Apoptosis Myocardial cells
CLC: R541
Type: Master's thesis
Year: 2008
Downloads: 36
Quote: 0
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Abstract


Objective To study the rat ischemia-reperfusion (Ischemia / reperfusion, I / R) calcineurin (calcineurin, CaN) changes in expression and apoptosis in cardiac myocytes and carvedilol (carvedilol) intervention on myocardial The rate of apoptosis and calmodulin phosphatase levels. Explore calmodulin phosphatase and relationship to apoptosis and carvedilol on the prevention and treatment of myocardial protection. Methods Adult male Wistar rats 36, were randomly divided into A and B groups, A group was divided into: sham operation group A, I / RA group, carvedilol pretreatment group, B group is divided into: sham surgery B group, I / RB group and carvedilol treatment group, n = 6. Pseudo-operation group A, group B and the I / R group rats were given saline 5 ml · kg -1 · d -1 , carvedilol the Los pretreatment and treatment groups given carvedilol 20 mg · kg -1 · d -1 . A group of animal gavage 7 days after the I / RA group and carvedilol pretreatment group ligation of the left anterior descending coronary branch 30 min reperfusion for 3 h, the sham group A only stringing without ligation. Group B first proceed as follows: I / RB group and carvedilol treatment group ligation of the left anterior descending coronary branch 30 min reperfusion for 7 d sham group B only threading, without ligation. Reperfusion for 7 d given orally. Reach the appropriate point in time, remove the myocardium samples by RT-PCR assay the expression levels of myocardial CaNmRNA Cardiomyocyte apoptosis was detected by flow cytometry (flow cytometry, FCM) rate. Results The two groups (A, B), respectively, for within-group comparisons. I / R, A, B the two groups CaNmRNA the expression levels were increased cardiomyocyte apoptosis rates were also increased. Carvedilol pretreatment group CaNmRNA expression levels compared to the I / RA group was significantly decreased (0.704 ± 0.025 vs 0.909 ± 0.016, P <0.05), myocardial apoptosis rate decreased (5.5% ± 0.9% vs17.3 % ± 1.0%, P <0.05). Carvedilol treatment group CaNmRNA expression levels than I / RB group decreased significantly (0.631 ± 0.014 vs 0.936 ± 0.025, P <0.05), myocardial apoptosis rate was significantly lower (7.6% ± 1.6% vs19.8% ± 1.8%, P <0.05). Conclusion of CaN expression in rat myocardial I / R enhanced apoptosis rate increase, suggesting that CaN play a role in promoting myocardial apoptosis. Carvedilol prevention and treatment interventions CaNmRNA expression and myocyte apoptosis rate decreased. Card Ming Kawei carvedilol can be achieved by reducing the expression of CaN I / R myocardial protective effect.

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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease
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