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Effect of Intrathecal Midazolam on c-fos Expression in Dorsal Horn of Spinal Cord in a Rat Model of Peripheral Pain

Author: HaoLiJun
Tutor: ChengQiao
School: Shanxi Medical
Course: Anesthesiology
Keywords: Midazolam Formalin Pain Genes, fos Injections, Spinal
CLC: R402
Type: Master's thesis
Year: 2008
Downloads: 40
Quote: 0
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Abstract


Objective To study the spinal cord antinociceptive effect in the rat model of formalin-induced pain Intrathecal midazolam median effective dose (ED 50 ) and c-fos expression in the dorsal horn of the spinal cord impact to explore midazolam dose analgesia in formalin-induced pain rat model of spinal cord level, to provide new ideas for spinal administration. Methods The experiment is divided into two parts (1) sequential determination of intrathecal injection of midazolam on formalin-induced pain in rats spinal analgesia ED 50 values. (2) intrathecal injection of midazolam on formalin-induced pain rat spinal cord c-fos expression. Select intrathecal catheter successfully 32 male SD rats were randomly divided into four groups (n = 8): control group (C group), saline group (NS), before the pain caused by the administration group (M 1 group) and cause pain after administration group (M 2 ). The intrathecal catheter 5d, NS group the the M 1 the group \u0026 M 2 rats in the left rear paws subcutaneous injection of 5% formalin 100μl induced pain 10 min before the pain caused by NS Group intrathecal injection of 20μl saline, M 1 group intrathecal injection of midazolam 22 μg (the ED 50 values ??and 95% CI OK) M 2 10min group on the pain caused by intrathecal injection of midazolam 22 μg (ED 50 determine the value and 95% CI), group C intrathecal injection of 20μl saline enough the bottom injection of 100μl saline as a control. Rats flinching, licking the claw-time and as a behavioral observation indicators and after injection of the plantar 2h All animals were sacrificed spinal cord enlargement Department, immunohistochemical method to observe the spinal cord dorsal horn fos-like immunoreactivity (FLI) positive cells in the spinal cord on the changes. Results (1) stability in the formalin-induced pain in a rat model of intrathecal injection of imidazole semi-effective when spinal cord antinociception of effective dose (ED 50 ) 21.8μg; (2) the rats in each group flinching, licking cumulative time comparison, NS group, M , 1 the group M 2 group flinching, licking cumulative time was significantly longer in group C (P <0.01); the the M 1 the group M 2 group flinching, licking the accumulated time is shorter than the NS group (P <0.01); and M 1 group is shorter than the M 2 group (P <0.01); (3) Group C, the NS group spinal cord dorsal horn c-fos expression was increased (P <0.01 c-fos expression dorsal horn of the spinal cord); compared with NS group, M 1 group M 2 group lower (P <0.01); M 1 the group M 2 Group spinal cord dorsal horn c-fos expression was increased (P <0.01). Conclusion (1) in formalin-induced pain in a rat model of intrathecal injection of midazolam in the spinal cord antinociceptive effect half way effective dose 21.8μg, the analgesic effect is more precise. (2) midazolam preemptive analgesia effect.

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