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Studies on the Separation of Ursolic Acid and the Antioxidative Activity of the Extract of Leaves of Eriobotrya Japonica

Author: ZhangJing
Tutor: WangHongXin
School: Jiangnan University
Course: Of Food Science
Keywords: Loquat Extract Separate Ursolic acid Oxidation resistance
CLC: R285
Type: Master's thesis
Year: 2007
Downloads: 409
Quote: 5
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Abstract


The the loquat Zhikepingchuan traditional Chinese medicine, lung, and the effect of the Heweijiangni. China is the world's leading loquat producing countries, the cultivation area and production accounting for more than 2/3 of the world, mainly distributed in the provinces south of the Yangtze River. In recent years, the study found that the loquat in triterpene acids have anti-inflammatory, hypoglycemic, anti-viral and anti-cancer activity, ursolic acid as the main ingredients of medicinal. This test as raw material to extract the essential oil and aqueous extract of loquat, explore new technology to extract separation of ursolic acid, to provide a reference for the comprehensive utilization of loquat. Extracted through the use of dissolved impurity, acid-base enrichment, separation and purification methods macroporous resin technology, and recrystallization, ursolic acid product purity of 95.10%. The main content of the study are as follows: (1) determine the RP-HPLC optimal conditions for accurate determination of ursolic acid content in loquat raw materials. Test by the choice of column and mobile phase adjustment, making the chromatographic peaks almost complete separation of stable baseline, peak shape, a quick and accurate determination of ursolic acid in loquat raw analytical methods. (2) investigated the solvent extraction extraction solvent, extraction time, extraction temperature, solid-liquid ratio as well as the number of extraction of raw materials extracted loquat ursolic acid yield. The optimum extraction conditions for the extraction solvent: 95% ethanol; extraction time: 2 h; temperature: 80 ° C; solid-liquid ratio: 1:14; extraction times: 2 times in this condition, the extraction of the loquat raw materials in Bear acid yield 1.150%. (3) Study on the process conditions of the water and petroleum ether to remove impurities in the ethanol extracts. Experimental results show that the amount of water is 1:50, and the amount of petroleum ether was 1:60, and repeated three times, to better remove the water-soluble and fat-soluble impurities in the ethanol extract. Again dissolved by the ethanol extract ursolic acid purity of 14.41% and 95.36% recovery rate. (4) to study acid-base purified enrichment ursolic acid conditions. Optimum enrichment conditions for the last determined: the pH of the solution was extracted with 10% NaOH basified = 14, then to 5% H2SO4 was acidified to adjust pH = 3 with 1 times the volume of sample solution pH = 3, the acidic aqueous solution was diluted to give ursolic acid product purity and recovery of 26.33% and 88.10%, respectively. (5) selection of a non-polar resin XDA-200 adsorption separation and enrichment solution ursolic acid. Adsorption efficiency up to finally arrive at the optimum process parameters: adsorption flow rate of 1.5 BV / h, the sample solution is a concentration of 3 mg / mL 96.00%; optimized combination of eluent eluted the elution flow rate of 1 BV / h , pH value of 11 90% ethanol afforded ursolic acid, eluent used in an amount of 6 BV. Three crystal ursolic acid product purity of 95.10%. Crystal melting point and optical rotation determination, thin layer chromatography, high performance liquid chromatography, infrared spectroscopy, and mass spectrometry for structural verification, and comparison with the standard of ursolic acid, can determine the for ursolic acid monomer purity greater than 95%. Throughout the process, the the ursolic acid total recovery of 31.40%. (6) the role the iodometric method loquat leaf extract of raw materials antioxidant. Extracts of the different stages of the antioxidant 2,6 - di-t-butyl-methylphenol (BHT) and tert-butylhydroquinone (TBHQ) added to the fresh lard, forced oxidation, and determination of the timing of oil samples peroxide value, and finally concluded that: compared with BHT and TBHQ, loquat leaf extract of raw materials has a weak antioxidant effect.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
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