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Objective: This experiment Wistar 3 to 4-month-old male rats to study, observe type 2 diabetic rat kidney cells intracellular calcium concentration ([Ca] i) and mitochondrial calcium mitochondria, cytochrome C (Cytc), succinate dehydrogenase (SDH), myeloperoxidase enzyme (MPO), xanthine oxidase (XOD), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Group C two Tsuen (MDA) levels and the expression of Bcl-2, Bax, apoptosis, expression, and Ganoderma lucidum spores of these indicators and the protective effect and mechanism of kidney damage in diabetic rats. Methods: Wistar male rats to 50, were randomly divided into normal control group (NC, 12 rats), model group (38). The rats were fasted for 16 to 18 hours, free access to water, the model group the 25mg/kg dose single intraperitoneal injection of streptozotocin (2% STZ), normal control group (NC) by intraperitoneal injection of the same amount of sodium citrate - citric acid buffer. Normal diet two weeks after tail vein measured fasting blood glucose, blood sugar is retained, I remove the retained under the rat diabetes mellitus (DM) group (14) and then randomly divided into treatment and Ganoderma lucidum spores (GL) group (13), and to feed the high-fat high-sugar diet, GL group plus Ganoderma spore powder (250mg/kg · d) continuous feeding of 10 weeks, the rats were killed, and quickly removed the kidney at low temperatures, determination of the various relevant indicators of change. Results: type 2 diabetes model was successfully. DM group kidney tissue apoptotic cells increased significantly, significantly higher than the NC group and the GL group (P lt; 0.05). DM group kidney tissue Bax protein expression was significantly more than the NC group and the GL group, Bcl-2 protein expression is reduced compared with NC group and GL group (P lt; 0.05). Bcl-2/Bax in the DM group compared to NC group and GL group was significantly reduced (P lt; 0.05). DM group kidney mitochondrial cytochrome C and mitochondrial calcium, mitochondrial SDH, SOD, GSH-Px water were significantly lower than the NC group and GL group (P lt; 0.05), cytoplasm Cytc [Ca2] i, MDA, MPO The XOD all significantly higher than the NC group and GL group (P lt; 0.05). Conclusion: The increased apoptosis rate of type 2 diabetic rat kidney mitochondrial pathway of apoptosis may be involved in the regulation of apoptosis in the kidney; Ganoderma spore powder by reducing kidney cell calcium overload, stability of mitochondrial calcium storage function change of Bax and Bcl -2 proportion inhibiting Cytc release caused apoptosis, and enhance the antioxidant capacity of kidney tissue, reducing the formation of free radicals and reduce free radical injury, has a protective effect on the kidneys of diabetic rats. Ganoderma spore powder the assistant drug as a treatment for diabetic nephropathy, has a very broad prospects for clinical application.
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