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Oujie (Nodus rhizomatis nelumbinis) for the dry roots of the water lily plant lotus (Nelumbo nucifera Gaertn) section of the Ministry, its medicinal has a long history in our country. \Modern clinical applications, its chemical composition analysis, Oujie promote the study of the the screening its mechanism of action of the clotting activity of substances have not been reported. The purpose of this paper is under active guidance, compare different solvent Oujie of early extracts pharmacodynamic differences and Oujie, in the initial extract has procoagulant track separation, hoping to obtain the effective parts of procoagulant and its mechanism of action is a preliminary study. The paper first analysis, the results showed that the chemical composition Oujie powder: the multiple intersecting powder may contain steroidal triterpenoids, volatile oils, phenols and tannins, organic acids, alkaloids, flavonoids, sugar and its glycosides saponins, proteins, cardiac glycosides, amino acids and other chemical constituents found no grease, anthraquinone, lactone and coumarin ingredients; rich the multiple intersecting powder nutrition content of various nutrients to moisture 1.57%, 7.47% of the total ash, crude lipid 4.62%, 9.58% crude protein, amino acids, 5.44%, and contains the body essential elements of potassium, calcium, sodium, magnesium, iron, manganese, zinc, copper and other minerals; gallic acid as a standard, determined by spectrophotometry Oujie powder total phenol content of 5.79%. Petroleum ether, 50% ethanol, water, 50% methanol and 50% acetone five solvents Oujie extracted the extracts obtained five Oujie early, through coagulation experimental determination of clotting time (CT), the activated partial thromboplastin The pharmacodynamic evaluation time (APTT), prothrombin time (PT) and thrombin time (TT), on five Oujie early extracts procoagulant OK solvent the multiple intersecting promote of coagulation active substances extracted. Experimental results show that Oujie promote coagulation active substances extraction solvent is 50% acetone. The system solvent method acetone Oujie early divided into pharmacodynamic evaluation extract parts of ethyl acetate, n-butanol extract fractions extracted water soluble parts, take the same pharmacological experiments of three extraction sites procoagulant extracts screening its the procoagulant effective parts. The experimental results show that the effective parts of the multiple intersecting procoagulant extract parts of ethyl acetate and n-butanol extract fractions, and the two effective parts of qualitative research, found that the ethyl acetate extract fractions may contain steroidal triterpenoids, phenolic n-butanol and the chemical composition of the tannins, alkaloids, flavonoids, sugar and its glycosides, the chemical composition of the extraction site may contain phenols and tannins, flavonoids, cardiac glycosides, sugar and its glycosides. Determination Oujie ethyl acetate extract fractions and n-butanol extract parts of the APTT, PT, TT, fibrinogen content (FIB) and platelet aggregation, and found two extraction site are able to significantly shorten the APTT, PT, TT and increased plasma FIB content. Prima facie, Oujie procoagulant mechanism through various coagulation factors play a role in the activation of endogenous coagulation system and the extrinsic coagulation system.
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