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Basic Study of Photosensitive Functional Azopolymers as the Adsorption Materials of Protein

Author: ZuoLiJun
Tutor: FengChuanLiang；ZhangZhiHong
School: Zhengzhou University of Light Industry
Course: Materials Science
Keywords: Azobenzene Optical isomers Atom transfer radical polymerization Plasma surface resonance instrument Biosensor Conductive polymer
CLC: O631.3
Type: Master's thesis
Year: 2010
Downloads: 57
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Abstract


Azobenzene compound has unique optical trans-cis isomerization characteristics, the the isomerization transition process will be accompanied by some of the physical and chemical nature of the change. Azo-based material is widely used in the controlled release of the drug, the nonlinear optical element, a liquid crystal material, a light regulation biologically sensitive material or the like. The papers prepared three kinds with different end-functionalized azobenzene polymers, and the use of Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (1H NMR) characterization of the structure, a detailed study of bovine serum albumin (BSA) (BSA) containing the azobenzene sensitive membrane adsorption behavior. The purpose of this project is to study the light, under the influence of two outfield BSA reversible adsorption mechanism in the the azobenzene polymer membrane surface for further research and development of photosensitive sensor chip to provide a theoretical basis. First, examine the BSA in the adsorption behavior of poly-4 - chloro-4'-azobenzene methacrylate (PMAAzoCl) film surface. The radical polymerization prepared PMAAzoCl, and the ultraviolet absorption spectrum (UV) optical isomers of the detailed study of the polymer characteristics in the UV - visible cycle under irradiation, PMAAzoCl isomerization transition occurs, and the light response speed , photoisomerization reaction to the first-order kinetic reaction. Next, by spin-coating technique in the preparation of the polymer film of the gold film and the silicon surface, and wettability change of the contact angle meter (CA) to examine the surface of the polymer film used in the UV - visible alternately irradiated. Reign research BSA adsorption kinetics of the surface of the polymer film, focusing on research in the UV - visible light cycle irradiation, the impact of changes in the structure of the polymer on protein adsorption using surface plasmon resonance instrument (SPR). SPR test results show that in the polymer film surface BSA molecule can undergo reversible adsorption. In order to investigate the adsorption of the polymer chemical structure of biomolecules, and the use of surface-initiated atom transfer radical polymerization (SI-ATRP) in the gold film and silicon surface preparation a regular structure azobenzene polymer brush, that poly - 4 - (6 - hydroxy-hexyloxy)-azobenzene-4'-carboxy-methyl acrylate (PMAAzoCOOH). Synthesized in the gold film and the silicon wafer surface grafting reaction required two initiator agent 6 - mercapto-2 - bromo-2 - methyl-propionic acid hexyl ester and N-(3 - methoxy silicon) n propyl-2 - Bromine - isobutylamide, and its chemical structure was characterized by FT-IR and 1H NMR, then assembled into a sequin and silicon surface, the SI-ATRP prepared azobenzene polymer brush. Then, using atomic force microscopy (AFM) to observe the polymer brush surface morphology changes before and after the UV - visible light irradiation. On this basis, the use of SPR and reign investigated Dynamic adsorption of BSA at the surface of the polymer film, the results showed that in the UV - visible effect, the polymer film can also achieve a reversible adsorption of the light control reversible BSA molecule. Finally, in order to further study of the field on the adsorption behavior and mechanism of biological molecules in azobenzene polymer surface by cyclic voltammetry (CV) and SPR investigated BSA photoelectric dual responsive polymer membrane surface reversible adsorption behavior . In order to obtain a dual response substance, the subject is introduced into the pyrrole molecule azobenzene substituents, and using a chemical oxidative polymerization to prepare a poly-4 - (6 - hydroxy-hexyloxy)-4'-carboxy azobenzene methacrylate polymer (PPyAzoCOOH), using UV and detailed study of the phenomenon of optical isomers of the polymer, compared with the above-mentioned two polymers PPyAzoCOOH photoisomerization rate significantly slower; simultaneously in the UV - visible alternately irradiated under AFM observation of BSA adsorption adsorption on the surface of the polymer film and solution. The CV test polymer film conductive performance and BSA polymer electrochemical activity of the composite electrode before and after adsorption, use of the SPR detection BSA polymer the reversible membrane surface adsorption kinetics, the results indicate that the azobenzene pyrrole polymer has a good photoelectric response characteristics. The optical isomers of the comparison of the three azobenzene polymer system can be found, with increasing chain length of the flexible spacer of the azobenzene molecule, optical isomers rate is reduced, while the function of the polymer of the azobenzene group types and field conditions affect the main reason for the adsorption of BSA.

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CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Polymer physics and physical chemistry of polymers > The chemical nature of polymers
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