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Spirulina polysaccharide characteristics the reaction BaCl2-gelatin method commonly used in the determination of sulfate group content measurement conditions made more systematic. BaCl2-gelatin method for the determination of the content of SO42-standard curve preparation conditions: K2SO4 as a standard, linear range of 0 to 40 microg system for the determination of HCl concentration of 0.10 mol / L, the determination of a wavelength of 360 nm. When the reaction temperature and time, respectively, in the range of 10 ~ 40 ℃ and 10 ~ 40min BaCl2-gelatin Determination of the content of SO42-. Centrifuge tube, 15N measuring tube and ampoules for the hydrolysis device Spirulina polysaccharide sulfate group content repeatability and accuracy of the measured values ??for the evaluation of indicators, selected Spirulina polysaccharide hydrolysis container and hydrolysis temperature and time of a single factor. Identified by means of hydrolysis, the hydrolysis temperature and time were 95 ° C and 4 h ampoules. BaCl2-gelatin method improved determination of the sulfate group content of 5 Spirulina polysaccharide with an average of 1.17%, the relative standard deviation (RSD = 0.08%), side built data reproducible, high credibility. Extraction of polysaccharides of Spirulina has been optimized extraction rate of indicators, in accordance with the principles of the spherical design. The results show that, when the process for the ratio of water to 30 ml / g, 74 ° C extraction 6h, extraction was better theoretical extraction rate of 3.89%. Spirulina polysaccharide extracted according to the improved process conditions of 4.02%, similar to the theoretical value of the average extraction rate of 3 replicates. The crude extract was concentrated to 5 times, polysaccharide recovery, purity and content of sulfate groups for the technical parameters, the system of alcohol precipitation pH and alcohol precipitation time single factor. The results showed that when the alcohol precipitation recycling system pH of about 3.5, the precipitation time was 18h, although the polysaccharide recovery is slightly lower, but the purity of the recovered polysaccharide sulfate group content are relatively high. Considering the removal rate of protein and polysaccharide recovery and sulfuric acid group content and other technical indicators, and TCA method of removing protein by Sevag method, comparative analysis, the study established a sulfated polysaccharide extracted aqueous extract of Spirulina The deproteinized method. Deproteinized Sevag method 2, the protein removal rate of approximately 51%, polysaccharide recovery rates and polysaccharide sulfate group content of 76.3% and 1.3%, respectively. Promise to increase the number of deproteinized are able to further remove contaminating proteins, but the sulfate group content decreased significantly. Collection deproteinized polysaccharide (SPS-CE), Q Sepharose Fast Flow column further isolated four sulfate group content were 20.7%, 11.7%, 8.2% and 13.0% of the sulfated polysaccharide component, denoted as SPS- Ⅰ, SPS-Ⅱ, SPS-Ⅲ and SPS-IV which SPS-I and SPS-IV. High purity, 90.6% and 84.6%, respectively. IR spectral characteristics analysis showed that the SPS-I in a sulfuric acid group is located in the vertical position of the monosaccharide residue C-4 position, and the other three SPS sulfate group on a monosaccharide residue key connection position and type vary different. Free radical scavenging experiments show that four kinds of SPS showed some antioxidant activity in vitro. Which, the strongest the SPS-IV resistance to the oxidation capacity, on · OH, O2-and 1O2 semi-scavenging capacity (IC50) 48.7,22.1 and 66.7μg/ml, is the SPS-CE of 3.36,33.1 and 2.76 times, respectively, Spirulina polysaccharide main antioxidant component. In view of the relationship between the radical with tumorigenesis and metastasis, which may be able to prompts from an aspect of the SPS-IV may have anti-tumor efficacy. Therefore, the authors further Pyricularia oryzae model SPS anti-tumor activity, a preliminary screening. The results show that the SPS-IV in a certain concentration range (187.50 ~ 375μg/ml) rice blast fungal mycelial growth the strongly deformed activity, and showed inhibitory activity with increasing concentration (gt; 750μg/ml). The results suggest that with SPS-IV is a potential anti-mitotic agent, with the ability to inhibit tumor cell mitosis barrier harm. In addition, the auxiliary analyzed by SAS software, explore the relationship between the SPS antioxidant and anti-rice blast mold activity and molecular characteristics. The results showed that the biological activity of SPS is not a decision to a single factor, but by the monosaccharide composition of sulfate group content and spatial conformation of many factors.
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