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Screening of High Producing Strain of Avermectin B1a Component

Author: LiYanXia
Tutor: QiaoJianJun；WangYongJun
School: Tianjin University
Course: Pharmaceutical Engineering
Keywords: Avermectin B1a component Directed Breeding Culture conditions Fermentation conditions
CLC: TQ465
Type: Master's thesis
Year: 2007
Downloads: 112
Quote: 0
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Abstract


Thesis avermitilis , has the use of ultraviolet light and nitrosoguanidine starting strain 1-17 mutagenic treatment , in combination with L- isoleucine induced by means of directional filtering last B1a component yield mutant 3-6, avermectin Bla yield than the original strain increased 12.86% . The subculture experiments show the strains of high-yielding and stable performance . Optimize the culture conditions of high-yielding strains 3-6 , the results showed that: slant medium ingredients yeast powder 's origin , slant seed cultivation cycle tampons weight of slant seed quality and fermentation results analysis, optimal yeast powder origin Hubei, best cultivation cycle 8d, optimum tampon weighs 7g . By orthogonal experiment to optimize the fermentation medium , the fermentation production can be increased by 7.35% . Also, the precursor substance is added sodium propionate fermentation results , the results showed that the fermentation process in the culture medium by adding a certain concentration of the precursor material sodium propionate, can significantly increase the fermentation yield , wherein, in the fermentation 24h 2.5mmol / L sodium propionate precursor substance added to the medium , the fermentation production can be increased by 9.89% . The effects of high-yielding strains 3-6 in 3 tons of seed tank and 100 tons fermenter metabolic properties, and its optimized fermentation conditions . The results showed that : The inoculation conditions through the selection and adjust the temperature of the seed tank 30 ° C the improvement measures to optimize the process of the seed tank ; through the adjustment formula of the base material , the adjustment of the amount of feeding scheme adjustment and ventilation optimize the process of the fermenter , such that the overall fermentation has been greatly improved , fermentation unit , total cans batch fermentation , fermentation index increased by 14.81% , 7.77% , 6.92% .

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