Dissertation > Excellent graduate degree dissertation topics show

The Research on Extraction of Natural Hormone from Antler Velvet with Supercritical CO2

Author: XuZhiHong
Tutor: LiShuFen
School: Tianjin University
Course: Pharmaceutical Engineering
Keywords: Supercritical CO2 Extraction Deer antler Natural sex hormones Estradiol Progesterone Polysaccharide
CLC: TQ461
Type: Master's thesis
Year: 2007
Downloads: 162
Quote: 2
Read: Download Dissertation

Abstract


The antler is traditional precious Chinese medicine, efficacy Warming impotence, Yijing blood, gluten bone health. Deer antler contains a variety of active ingredients contained in the natural sex hormones, such as estrogen and progesterone, can be used to treat the symptoms of menopause in women before and after menopause. Isolated the active ingredient rich in natural sex hormones extracted from deer antler, clear effects to the development of new drug safety prevention and treatment of women with menopausal symptoms has important academic significance and practical value. In this paper, on the basis of the results of the research group preliminary studies, the first using supercritical CO 2 extraction technology, a more systematic study of the conditions of estradiol and progesterone extracted from deer antler. In this study, the total extraction rate of estradiol and progesterone, and their respective content in the extract as an objective function, the entrainer concentration of entrainer, extraction pressure and extraction temperature factors were investigated, the experimental results show that: when with 85% ethanol solution entrainer their and velvet than 1 (mL / g) static add, extract the total extraction rate of up to 1.56%, estradiol and progesterone content in the extract were 3.07ng / g, 776.18ng / g; 85% ethanol solution for dynamically added entrainer 15 (mL / g) ratio, and then extract the total extraction rate of up to 3.88%, respectively estradiol and progesterone extract is 3.86ng / g, 588.34ng / g. Article also examined the four traditional extraction methods (thermal reflow method, ultrasonic method, oscillation method and Soxhlet extraction), with the supercritical CO 2 extraction techniques were compared and found to dynamic The supercritical CO cosolvent 2 extraction has certain advantages. Carried out a systematic study of the chemical composition of the extract in this article at the same time using a variety of analytical methods, including: detection by radioimmunoassay of estradiol and progesterone levels; GC-MS analysis of the extract lipids; neighbor phthalaldehyde colorimetric analysis of the cholesterol content; thin layer chromatography (TLC) qualitative analysis of the phospholipid component; HPLC analysis of the extract content hydroxybenzaldehyde and hypoxanthine. The establishment of the above analysis method to provide the necessary basis for the application data for process control and product quality. In addition, using hot water extraction, dilute alkali extraction, dilute alkali - enzymatic hydrolysis of the extract of deer antler supercritical CO 2 extraction residue of polysaccharide preliminary study results show that the dilute alkali - The enzymatic method is an effective way of comprehensive utilization of velvet.

Related Dissertations

  1. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  2. Analysis of Nutritional Compositions and Quality of Shishen (Eremurus Chinensis Fedtsch.),S647
  3. Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
  4. Study on Synthesis of Polysaccharide Tris (Phenylcarbamates) Derivatives as Chiral Stationary Phases (CSPs) and Estimation of Their Resolution Abilities,TQ460.1
  5. The Supercritical Carbon Dioxide Extraction and Functional Quality Evaluation of Camellia Seed Oil,TS225.16
  6. The Influence of Antibiotics on Yogurt During the Fermentation,TS252.54
  7. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  8. Cloning and Fuctional Study of clp Gene of Xanthomonas Oryzae,S435.111.4
  9. Effects of Kisspeptin-10 on Progesterone Secretion of Chicken Ovary Granulosa Cells and Mechanism Research,S831
  10. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  11. Fundamental Studies on the Chemical Constituents of Brassica Rapa L.seed.,R284
  12. Secreening of Harpin-like Genes and Identification of Extracellular Polysaccharide-Associated Genes in Xanthomonas Oryzae Pv. Oryzicola,S435.111.42
  13. Development of the Colloidal Gold Immuno-Chromatographyic Strips for the Early Pregnancy Diagnosis of Hu Sheep,S858.26
  14. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  15. Pharmacokinetics of Progesterone Cream in Beagle Dogs Determined with LC-MS/MS,R96
  16. Study on the Extracting Technology of Polygonatum Polysaccharides and Preparation of the Polygonatum Sibiricum Yoghurt,TQ461
  17. The Study on Extraction and Purification of Polysaccharides and Cordycepin from Cordycepin Militaris,TQ461
  18. The Research of Tea Seed Polysaccharide from Cold Squeeze Leaching Camellia Cake,TS229
  19. The Correlation of Ultrasound Elasticity with the Expressions of Estrogen Receptor, Progestron Receptor, C-erbB-2 in Breast Cancer,R445.1
  20. Plateau rape pollen extraction and analysis of fatty acids,S896.4
  21. Purification and Identification of an Acidic Polysaccharide Fraction GFLA-1 from the Flowers of Panax Ginseng C. A. Meyer,S567.51

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > The Chinese herbal medicine preparations of the production
© 2012 www.DissertationTopic.Net  Mobile