|
Molecularly imprinted polymers (molecularly imprinted polymers, MIPs) is the specific adsorption of the target molecule (template molecule) synthetic polymer compound. MIPs can be specific recognition and binding target molecules stable physical properties, organic solvent, acid and heat resistance, and thus has a broad application prospects in the separation and detection of trace organic pollutants. Molecularly Imprinted Polymer various precipitation polymerization is characterized by its simple preparation of controlled particle size, higher yield, imprinting is very effective as the most common polymerization method. However, the conventional precipitation polymerization requires the use of a large number of porogen (approximately 95% of the total volume), likely to cause environmental pollution and the costs increase. In order to overcome the shortcomings of conventional precipitation polymerization method, the laboratory first established improved precipitation polymerization (modified precipitation polymerization, MPP). The papers to the environmental estrogen estradiol as the template molecule, using the modified precipitation polymerization method the low dispersity MIPs microspheres, and the system of the polymerization conditions of MIPs microsphere morphology and adsorption properties, to optimize the preparation conditions. Then the adsorption properties of the MIP as stationary phase for solid-phase extraction, separation and enrichment of estradiol in the biological and environmental samples, combined with the HPLC method, determination of trace amounts of estrogen in the chicken, orange juice, fish, pigs, water diol. The work of this paper includes the following two aspects: First, to estradiol as the template molecule by precipitation polymerization synthesis of molecularly imprinted microspheres research imprinted polymer selectivity and binding characteristics through the of selective experimentation and frontal chromatography, impact. To adsorption optimal performance estradiol molecularly imprinted microspheres as a solid-phase extraction stationary phase, the separation and enrichment of trace amounts of estradiol in the chicken, orange juice, fish, pigs, water. First, to optimize the balance, loading, washing and elution solid phase extraction step, the optimal solid phase extraction conditions, solid phase extraction column to selectively identify, capture and enrichment of 0.5-10μmol / L standard mixed standard estradiol recovery solution between 71.16% -89.26%, intra-day and inter-day RSD less than 2.6% and 1.73%, respectively. Spiked chicken, orange juice, fish, pigs, water recoveries were 71.16%, 83.75%, 75.25%, 74.98% and 89.26%, respectively. Better separation and recovery relative to C18 solid phase extraction column, estradiol molecularly imprinted column extraction liquid was detected by HPLC. The study suggests that improved estradiol has a strong affinity to the template molecule and selective precipitation polymerization synthesis of estradiol molecularly imprinted microspheres offline enrichment marks in a variety of environmental and biological samples The amount of estradiol, estradiol detection has practical applications.
|