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Construction of Eggplant Anther Culture System

Author: QiWeiQiang
Tutor: SunZhiQiang
School: Henan Agricultural University
Course: Olericulture
Keywords: Eggplant Anther culture Pretreatment Callus Adventitious buds Induction rate
CLC: S641.1
Type: Master's thesis
Year: 2008
Downloads: 144
Quote: 3
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Abstract

Four varieties of eggplant (green cans tomatoes (4) in the anther culture stage, the selection of green eggplant (6), aerospace Purple Crown (19) the new economic Miscellaneous eggplant 9th (29)) test material, its anther callus induction, callus proliferation and adventitious buds trial, the purpose is to build a more complete set of eggplant anther culture system. The test results are summarized as follows: 1 bud length, bud diameter, corolla length, the base of the calyx crack length, anther length, bud color, anther color, calyx degree of cracking the absolute value can be used as a supplementary indicators to determine the the bud shape with pollen The correspondence relationship between developmental stages, but is used to directly determine the correspondence relationship between the two is not reliable. Determine the the bud shape with pollen developmental stages between corresponding relationship is more reliable and straightforward method is to use the relative value of the difference between the lengths of the corolla length and the base of the calyx cleft, corolla length is less than the length of the base of the calyx crack, most of the pollen in tetrads period; corolla length greater than the length of the base of the calyx crack, and the difference between the 0 to 0.2cm, most of the pollen in mononuclear advanced; If the difference is greater than 0.2cm, most of the pollen is binucleate or mature. Two of the tested varieties 6 to 8d 36 ° C high temperature pretreatment can significantly improve callus induction rate, but the slow response of four varieties, 19 varieties are more sensitive response. Two tested varieties the 4d 4 ° C low temperature treatment and then 4 ~ 8 days of 36 ° C high temperature treatment the variable temperature pretreatment, the same can significantly improve the rate of callus induction. Two tested varieties of different temperature pretreatment reaction different varieties 4 short-term low temperature and high temperature heat treatment time can be shortened significantly, No. 19 varieties of variable temperature pretreatment effect but not as good as the single The effect of high-temperature pretreatment. Anther callus induction of two of the tested varieties most suitable combinations of hormones: 0.2mg / L 2.4-D 0.5mg / L ZT. In the role of this hormone combination, the two varieties tested in the 6th and the 29th callus induction rate reached 19.77% and 36.10%, respectively. 5 both callus induction or adventitious buds induced Add ZT medium induction rate than add KT medium induction rate significantly improve. Especially on the adventitious buds, add the KT medium induction rate all 0, and added ZT media in the four tested varieties adventitious bud formation, especially the four varieties, add two ZT a medium, 3 and 4, the rate of adventitious buds, respectively, 62.5% and 20%. The other three tested varieties in ZT Add medium adventitious bud induction is still not very high, and the best combination of hormones to be further study to determine. 6 genotype eggplant anther in vitro culture influence. This experiment, tested several varieties of different temperature pretreatment, different combinations of hormones (including callus induction and adventitious buds) reaction, showing a great difference in the induction rate. Comparison of each of the tested varieties callus induction, purple eggplant varieties on the 19th, the 29th induction rate was significantly better than the 4th and the 6th green and eggplant varieties, of which the highest on the 19th of the tested varieties callus induction 18.8%, and callus formation shortest time, and several other varieties of callus formation of a relatively long time. Callus proliferation effect, different genotypes showed significant differences, the proliferation of the 4 tested varieties the highest conversion rate reached 38.8%, the highest rate of callus induction 19 varieties in the proliferation and transformation rate but the lowest at 9.52%.

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