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Molecular Marker Screening for the New Powdery Mildew Resistance Gene in Lankao906

Author: WangBaoQin
Tutor: NiuJiShan
School: Henan Agricultural University
Course: Crop Genetics and Breeding
Keywords: Wheat Gene chip Powdery mildew Molecular markers SSR ESTs
CLC: S512.1
Type: Master's thesis
Year: 2008
Downloads: 33
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Abstract


Wheat Lankao 906 Series strains province to cultivate new germplasm resistant to powdery mildew. Genetic analysis showed that Lankao 906 carrying a small species of specialized recessive resistance to powdery mildew genes temporarily marked as PmLK906. In order to in-depth research and Lankao 906 powdery mildew resistance gene effective use of molecular markers linked closely with the PmLK906 screened with SSR markers and gene chip technology. This gene is located by the spring nullisomic - four system and deletion lines in wheat chromosome 2AL. The results can be directly used for molecular marker-assisted breeding, and laid the foundation for map-based cloning cloning resistance genes PmLK906. The specific contents and results of this study are as follows: 1 to susceptible varieties the Chinese Spring yumai 21, Pu 9 as the female parent, the preparation of resistant and susceptible crosses Lankao 906 male parent. Culture from leaves section on F 1 , the F 2 -generation segregating populations, F 2:3 Home Department of segregating populations seedling stage anti- disease phenotype that Lankao 906 strains carrying small kinds of specialized recessive powdery mildew resistance gene. To Chinese Spring / Lankao 906 hybrid F 2:3 home for materials, \SSR markers. Screened a total of 150 pairs of SSR primers, the two pairs the primer gwm265 and gdm93 stable polymorphism between the resistant and susceptible parents and resistant and susceptible DNA pools. 3, 2 pairs of primers in the 129 family of Chinese Spring / Lankao 906 hybrid F 2:3 home, segregating populations for linkage analysis and verification results show that the amplification products and gwm265 and gdm93 PmLK906 genetic linkage, genetic distance of 3.72 and 6.15 cM, this flag is recorded as Xgwm265 and Xgdm93 4 by Chinese Spring nullisomic - four system and deletion lines will be marked the Xgwm265 positioning on wheat chromosome 2AL, which will PmLK906 located in the 2AL. 5, Chinese Spring / Lankao 906, hybrid F , 2:3 Home Department, the establishment of resistant and susceptible cDNA pool with BSA method. As a probe to the anti-sense cDNA pool filter the wheat gene chip (Affymetrix products), with PmLK906 ESTs. On this basis, clone a similar genetic with Ae.tauschii csAtPR5, named TaAetPR5 6 linkage analysis and verification in the Chinese Spring / Lankao 906 hybrid F 2:3 pedigree segregating populations, the results that TaAetPR5 gene chain and PmLK906 genes, the genetic distance of 7.62 cm. Segregating populations in Chancellor / CI 14124 hybridization, linkage analysis proved, TaAetPR5 gene with Pm4a gene closely linked to the genetic distance of 0 cM.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
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