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Synthesis and Molecular Recognition of Flavonoids Functional Factors of Traditional Chinese Medicines-Imprinted Polymer

Author: GuoLiFei
Tutor: HeMinQiang
School: Jiangsu University
Course: Applied Chemistry
Keywords: Flavonoids Functional factor Molecularly Imprinted Polymer Adsorption
CLC: O631.3
Type: Master's thesis
Year: 2009
Downloads: 54
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Abstract


The molecular imprinting technique (Molecular Imprinting Technology, MIT) is a process for preparing a polymer material having a strong affinity and high selectivity of the target compound (template molecule) of the new technology. Preparation of polymer called molecularly imprinted polymers (Molecularly Imprinted Polymers, MIPs). Since MIPs as template to synthesize a specific target compound, and its position in the spatial structure and functional groups, is highly consistent with the template molecule, which has a memory to identify the ability of the template molecule and its structural analogues. This memory to identify with a high degree of selectivity and specificity. Molecularly imprinted polymers because of its unique nature, are widely used in the separation, analysis, catalysis, sensors, and other fields, very broad prospects for development. Chinese herbal medicine is a complex system of natural combinatorial chemistry library, the quality of Chinese herbal medicine relies heavily on Chinese herbal medicine in various functional factors separation / extraction effect. Therefore, to selectively separated from the complex system of herbal extract effective function factor has important application value and practical significance. In this paper, the basic principle of MIT, the preparation and application of MIPs were more comprehensive overview of the the MIT current problems and future trends and outlook. Herbal functional factors and two flavonoids (Apigenin, API) apigenin and luteolin (Luteolin, LUT) as a template molecule, prepared molecularly imprinted polymer bulk polymerization, the use of Fourier transform - infrared spectroscopy (FT -IR), ultraviolet-visible spectroscopy (UV-vis), thermal gravimetric analysis (TG-DSC), scanning electron microscopy (SEM), surface area and porosity analyzer such as their structural characterization. Investigated by spectrometry of non-covalent interactions between the template molecule and functional monomer, and examine the performance of the porogen, functional monomer species, the amount of monomer, crosslinker, polymerization temperature and other factors on the polymer adsorption optimized molecularly imprinted polymer synthesis conditions. By static adsorption experiments investigated the adsorption properties of the imprinted polymer and molecular selection performance imprinted polymer identification, mass transfer and adsorption mechanism was discussed. And evaluation by high-performance liquid chromatography (HPLC) the two imprinted polymer the API crude extracts of Chrysanthemum (Chrysanthemum morifolium Ramat) and LUT separation / enrichment effect. The main findings are as follows: 1. API template molecules prepared by bulk synthesis of molecularly imprinted polymers (API-MIP), and its structural characterization. API-MIP optimum synthesis conditions are: tetrahydrofuran (THF) as porogen, 2 - vinyl pyridine (2-Vpy) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as crosslinking agent, n (API) : n (2-Vpy) : n (EDMA) = 1:8:40, the reaction temperature was 60 ° C. 2 LUT for the template molecule prepared by bulk synthesis the corresponding molecularly imprinted polymers (LUT-MIP), and its structural characterization. LUT-MIP optimal synthesis conditions: tetrahydrofuran / chloroform mixed solution (THF / the CHCl 3 , v / v = 8:2) the ,2-Vpy porogen as functional monomer, EDMA as crosslinker. n (LUT) : n (2-vpy) : n (EDMA) = 1:10:50, the reaction temperature of 60 ° C. 3.API-MIP has a good affinity to the template molecule API and adsorption selectivity of its adsorption isotherms of Langmuir adsorption isotherm. Scatchard analysis of the results showed that: There are two different binding sites inside the polymer. High-affinity binding sites for the dissociation constant: K d1 = 99.01μg/mL, apparent maximum: Q max1 = 1165.05μg / g; low affinity binding sites The dissociation constant: K d2 = 2000.00μg/mL apparent maximum: Q max2 of = 21274.67μg / g. Kinetic studies showed that within the first 45 min, API-MIP adsorption fast, 2h saturated adsorption can be achieved. 4.LUT-MIP template molecule the LUT has good affinity and adsorption selectivity of its adsorption isotherms of Langmuir adsorption isotherm. Scatchard analysis of the results showed that: inside the polymer also has two different binding sites. High-affinity binding sites for the dissociation constant: K d1 = 312.50μg/mL apparent maximum: Q max1 = 1796.09μg / g; low-affinity binding site number constant: K d2 = 1111.11μg/mL apparent maximum: Q maxm2 = 4768.77μg / g Dan. Kinetic studies showed the previous 30 min, LUT-MIP adsorption fast, 1 h to reach saturation adsorption. TG (TG-DSC), scanning electron microscopy (SEM), surface area and porosity characterization shows that, the API-MIP and LUT-MIP are porous, strong thermal stability of the polymer. Renewable experiments show: two molecularly imprinted polymer can be repeated several times to take advantage of. 6 high-performance liquid chromatography (HPLC) were evaluated MIPs applied to the separation of the crude extracts of Chinese herbal medicine Chrysanthemum function the factor API and LUT / enrichment results show that these two polymers of the Chrysanthemum crude extract of API and LUT has a good separation / enrichment.

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CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Polymer physics and physical chemistry of polymers > The chemical nature of polymers
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