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In Vitro Method for Producting Milky Spore

Author: DingYing
Tutor: WangJinFeng
School: Tianjin University
Course: Medicinal Chemistry
Keywords: Japanese beetle Bacillus Microbial insecticides Spore Milky disease
CLC: S476
Type: Master's thesis
Year: 2007
Downloads: 48
Quote: 1
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Abstract


Scarab is currently one of the most harmful pest species in the world , countries in agriculture , forestry, animal husbandry and cause a great impact . Adult feeding habits very mixed, eat a variety of plant leaves , stems, leaves and fruit hazards ; larvae dwelling in the destruction of plant roots in the soil , leading to plant death . Therefore, how to effectively prevent and control the scarab has become the focus of biological control of . Japanese beetle Bacillus is an insect pathogen Metarhizium milky disease , made ??of the kinds of Bacillus spores microbial pesticide can be safe and effective control of the quantity and hazards of the chafers , the kind of bacteria , however , the conditions require very harsh, difficult outside the living organism to grow and produce spores . This paper mainly studied in a soluble starch, yeast extract, dipotassium hydrogen phosphate , sugars , calcium carbonate as a main component of the liquid medium in the production method of the Japanese beetle bacillus spores . Through the determination of the factors that affect the growth of bacteria living organisms outside and a spore , to find the best conditions and methods of beneficial bacteria growth and sporulation : will glucose as the sugar source pH value of 7.6, medium joined conical flask, inoculum size of 1% inoculum broth at 30 ° C and shaker of 200 rpm shaking bacteria 16h to adjust the broth pH value to 8.0 , adding MnSO4 and macroporous resin , and then at 30 ° C , 200 rpm shaker shake bacteria 24h lyophilized spore recovery . Using the above method to the generation rate of more than 70% of spores in living organisms . The meaning of the present research is to provide a large number of artificial medium the Japanese beetle Bacillus spores , laid the foundation for the mass production of the insecticidal components of the kinds of strains of microbial insecticides , has a very important significance to the biological control as soon as possible the transition from chemical control to promote the prevention and treatment of our chafers .

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control
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