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Study on TAT Mediated Magnetic Microspheres Having Composite Targeting Funtion

Author: LiShuangYan
Tutor: ChangJin；KangChunSheng
School: Tianjin University
Course: Materials Science
Keywords: Chitosan derivatives Magnetic targeting Transmembrane peptide Folic acid Blood-brain barrier Gene vector
CLC: R318.08
Type: Master's thesis
Year: 2007
Downloads: 107
Quote: 0
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Abstract


Chitosan modified through lysine, the amino group content of the chitosan is increased, improving the water-soluble under a neutral condition, and lysine are prepared in the different preparation process conditions modified chitosan, come lysine modified chitosan optimal conditions: stirring speed of 1200 rpm and a temperature of 20 ° C, oil and water, compared to 1, and the reaction time of 6 h. By the solubility of the product, the infrared absorption spectrum and NMR tests verify the solubility of lysine is chemically bonded to the chitosan above, and the drawn product is 0.48 g, the lysine substitution degree of 32.78%. Lysine modified chitosan prepared for the shell material, iron oxide nuclear magnetic ultrafine carrier using coprecipitation method. Transmission electron microscopy and laser particle analyzer analysis shows that the smooth surface of the carrier particles, the particle size distribution, to about 100nm; the VSM magnetic energy analyzer and Zeta potential instrument tests show that the carrier particles have good superparamagnetic and higher electropositive. Then connected to the transmembrane peptide TAT and folic acid (Folate) lysine modified chitosan the ultrafine magnetic carrier particle surface, to form a composite transmembrane and magnetic and receptor-mediated targeting function ultrafine magnetic carrier particles (TAT -FA-LCMNPs), using UV spectrophotometer for each component of the composite function carrier system quantitative analysis confirmed the success of the composite function ultrafine vector system build. On this basis, the process for the preparation of the four kinds of magnetic carrier particles: the lysine the modified chitosan ultrafine carrier (LCMNPs), folic acid mediated lysine modified chitosan the ultrafine vector (FA-LCMNPs), having a TAT cross lysine modified chitosan membrane function AMD the carrier (TAT-LCMNPs) and a composite targeting function ultrafine vector (TAT-FA-LCMNPs) by agarose gel electrophoresis, were determined with the DNA complex . Then labeled with 99mTc radioactive carrier particles, SD rats tail vein and carotid artery were injection and detection of single photon emission computed tomography (SPECT), investigated carrier particles carrying plasmid DNA through the blood-brain barrier circumstances and by different groups, different modes of administration, different magnetic field time and negative control group (radioactive elements 99mTc carotid injection) compared to determine the optimal treatment method. The results show that the administration of the carotid artery, the magnetic field acts on the rat head at 30min, DNA-TAT-FA-LCMNPs across the BBB has the best effect.

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