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Purpose : eczema sprays prepared by Cynanchum , flavescens , skullcap Chinese medicine clinically for the treatment of eczema have a significant effect . Conducted a systematic experimental study of the preparation process of the preparation and quality evaluation . Methods : ( 1 ) paeonol indicators preferred Cynanchum distillation collected optimum volume of distillate ; (2) as an indicator of the extract yield Matrine Sophora medicinal water extraction process research ; ( 3) as an indicator of matrine concentrate drying process of water extraction ; (4 ) as an indicator of baicalin and berberine hydrochloride content , examine the skullcap and Treats alcohol extraction conditions ; ( 5) the shikonin absorbance indicators , conduct research on the Lithospermum alcohol extraction process ; ( 6) in vitro skin method , the type and concentration of penetration enhancers screening ; ( 7 ) 5 taste herbs in the other side of the thin-layer chromatography identification test ; using high performance liquid chromatography method for the determination of paeonol . Results: Cynanchum , distillation, of 8 times distillate collected considerable Cynanchum weight ; Cynanchum dregs flavescens other herbs soak 1.0 h, were added to an amount of 8 times , 6 times the amount of water extracted 2 times , respectively 2.0H , 1.5h , the concentrate was spray dried. Scutellaria were added to an amount of 6 times , 4 times the amount of 70% ethanol and extracted twice , respectively 1.0H , 0.5H ; Huangbai were added to an amount of 10 times , 8 times volume of 80% ethanol and extracted twice , extraction time were 1.0h , 0.5h. Add 2.0% of the preparation process Azone . Preparation Cynanchum , Scutellaria , Phellodendron , Sophora , borneol identified using thin layer chromatography . HPLC Determination of paeonol and methodological study , the average recovery was 100.3 RSD = 0.60 % . Conclusion: The experimental formulation a reasonably practicable process and route , screening the best concentration of penetration enhancers and their use , and to determine the possible quality testing methods , so as to ensure maximum clinical efficacy of this compound .
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