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Purpose: the use of polypropylene mesh stent, preparation of silk fibroin coated polypropylene material, and its physical and chemical properties. Method: prepared silk fibroin solution coated in the the polypropylene mesh surface processing in the plasma environment, using scanning electron microscopy, infrared spectroscopy, Raman spectroscopy was used to detect physical and chemical properties of silk fibroin coated polypropylene material silk fibroin coated. Results: hydrophobic polypropylene patch in the atmosphere of air using the helium-containing plasma surface treatment can be to have a certain degree of hydrophilicity, conducive to the wetting of the silk fibroin solution, so that the silk fibroin solution may be coated coated polypropylene mesh to form a silk fibroin modified polypropylene material. Scanning electron microscope, the smooth surface film polypropylene fiber polypropylene fill unused silk fibroin coated, polypropylene mesh in the polypropylene monofilament tangles Office to form a silk fibroin silk fibroin solution coating film shaped infrared spectra of silk fibroin-modified polypropylene mesh 1650,1537 and 1242 cm ,, -1 silk fibroin random or α-helical conformation absorption peaks in the Raman spectra also corresponds to the absorption peak of the silk fibroin Conclusion: The use of plasma surface treatment of hydrophobic polypropylene mesh can improve the hydrophilic polypropylene mesh favor silk fibroin aqueous solution in polypropylene fill wetting and coating of the sheet surface, the plasma processing time is longer, the better the effect of the silk fibroin-coated. In the case of the strength of the material can be allowed, for two minutes of the plasma treatment, the surface of the polypropylene mesh silk fibroin can obtain better coating. Rat abdominal wall defect animal model of experimental research Objective: To establish the experimental rat abdominal wall defect model. Methods: 10 male SD rats removal of abdominal paramedian incision layers separating the abdominal wall layers of the structure to the parietal peritoneum and abdominal muscle tissue in the rat remnant 2 × 2cm, be hemostasis while preserving the abdominal wall, the surface of the skin and subcutaneous tissue January, postoperative feeding, the establishment of a general model of postoperative rats were observed, and confirm the abdominal wall defect area. Results: abdominal wall defect animal models produced basic food intake in rats after normal postoperative body temperature normal, basic 37.8 ~ 38.5 ℃, 3 abdominal wall defect animal model after minor local subcutaneous hematoma, improved after the group all mouse was no infection, all specimens observed January after abdominal wall defect animal models were produced successfully form a local defect in the abdominal wall, the measurement of the defect size (L * W) (2.15 ± 0.04) * (2.09 ± 0.02) cm < sup> 2 . Conclusion: The use of resection of the abdominal wall muscle tissue production purpose: To explore the silk fibroin coated polypropylene material role in the repair of abdominal wall defects and reduce postoperative complications. The polypropylene material group (20): random number table 60 male SD rats were randomly divided into silk fibroin coated polypropylene mesh (20), the ePTFE group of (20). By group were implanted silk fibroin coated polypropylene materials, polypropylene mesh and ePTFE patch, patch size 2 * 2cm, respectively, to repair abdominal wall defects, suture surgical wounds. After three days, one week, two weeks, and 12 weeks were sacrificed rats, and to observe the situation of the rats abdominal wall hernia repair patch implanted with or without deformation, parallel pathology and scanning electron microscope examination. Another two normal SD rats were excised abdominal wall defect area and the skin, subcutaneous tissue as detected by light microscopy and electron microscopy reference, and the experimental data obtained using SPSS16.0 software to analyze statistics. Results: silk fibroin coated polypropylene material group after three days, one week, two weeks, 12 weeks to check basic intra-abdominal adhesion formation; the histopathological examination polypropylene patch surrounded by granulation and scar formation, the surface of the patch mesothelial cell morphology typical reconstruction after 12 weeks fibroin is not completely absorbed, residual silk fibroin is biocompatible, scanning electron microscope examination showed mesothelial cells grow well, neat; already mesothelial cells after 3 days ingrowth, 1 week reduce the inflammatory response time mesothelial cells have completely covered the surface of the patch, 2 weeks and replaced by adipose tissue and neovascularization, the cross-sectional area of ??the patch surface adhesion total patch size (4.81 ± 4.50)%, hereinafter the same respectively after 2 ~ 9 d (x ± S)%; pure polypropylene group all rats postoperative intra-abdominal adhesions (74.96 ± 23.57)%, 5 (5/20, 25.O%) rats bowel, and polypropylene adhesions causing intestinal obstruction, intestinal fistula deaths, 12 (12/20, 60.0%) important organs in rats concurrent patch intraperitoneal adhesions, after three days, one week, two weeks, 12 weeks histopathological examination, polypropylene mesh around the obvious granulation and scar formation, the surface showed no typical mesothelial cells covering, two groups of postoperative intra-abdominal adhesions area and adhesion properties of the differences were statistically significant (P value <0.01); simple e-PTFE group all rats postoperative intra-abdominal adhesions (2.26 ± 1.61)%, the patch surface there was no significant adhesions, the histopathological examination see some typical mesothelial cells on the surface coverage, silk fibroin coated polypropylene materials group with a simple e-PTFE group postoperative intraperitoneal adhesion area and adhesion properties of the difference was not statistically significant (P> 0.01), three groups of postoperative complications of intestinal obstruction, intestinal adhesion induced intestinal The incidence of fistula, intra-abdominal infection there is a significant difference (P <0.01). Conclusion: the fibroin silk fibroin coated polypropylene material has good biocompatibility for SD rat peritoneal patch can provide enough tissue tensile strength and reduce intraoperative patch with intra-abdominal organs the magnitude and extent of adhesions can promote the ingrowth peritoneal mesothelial cells, on the polypropylene mesh surface of the peritoneum, the formation of a new layer of mesothelial cells, and is expected for Clinical various types of abdominal wall hernia repair.
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