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Lung Metastasis of Human Choriocarcinoma in SCID Mice: Establishment of Experimental Metastatic Model and Its Biological Characteristics

Author: ZhengJingHong
Tutor: XuCongJian
School: Fudan University
Course: Obstetrics and Gynaecology
Keywords: Lung metastases Choriocarcinoma SCID mice Model Experimental metastasis
CLC: R737.33
Type: Master's thesis
Year: 2008
Downloads: 83
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Abstract


The ideal the choriocarcinoma lung metastasis model is an important foundation for one of choriocarcinoma lung metastasis research. In this study, through the tail vein of SCID mice injected human choriocarcinoma cell line JEG-3, whereby the establishment of a more clinical features of choriocarcinoma lung metastasis in animal models. The first part based on the intravenous injection of tumor cells the choriocarcinoma in SCID mice lung metastasis model purpose: to establish a more consistent clinical features of human choriocarcinoma lung metastasis in animal models, and explore successful model suitable cell concentration. Methods: 40 4-5 week-old SCID mice were randomly divided into four groups (depending on the concentration of the injected cells are divided into four groups of A, B group, C group, D, n = 10), human choriocarcinoma made 1 × 10 7 cells / ml of the cell line JEG-3, 5 × 10 6 cells / ml, single-cell suspension of 1 × 10 6 cells / ml of three concentrations. The two concentrations of cell suspension was injected by the tail vein of SCID mice. A group each injected 0.1ml of 1 × 10 7 cells / ml of cell concentration; group B each injection of 5 × 10 6 cells / ml concentration of cells 0.2ml; C group per injection of 1 × 10 6 cells / ml concentration of cells in 0.1 ml of; D group is set to the normal control group. Every three days for observation of a mouse state, weighing a body weight of mice, with small animal imaging CT system checks the lungs when mice moribund state whether the placeholder and the number of the Micro CT examination, de vertebral killed SCID mice, systemic anatomy, visual observation of the various organs of the presence or absence of tumor foci and bilateral lung surface metastatic nodules in the number of specimens made of biopsy observed under the microscope. Results: A group: intravenous injection of all deaths; B group: intravenous injection of 3 deaths; survival 7 in 18 ± 2 days, moribund state; weight quickly in the first 12 days after inoculation JEG-3 cells rise, after rapid weight loss; Micro CT examination, 5/7 lungs to see the obvious placeholder 1-3 each lung space-occupying lesions, placeholder diameter 1.5-3.5mm; 1/5 of the naked eye see liver metastases, I SCID mice were no other organ metastases were not checked to see chest, ascites; biopsy confirmed choriocarcinoma. Group C: intravenous injection of two died; survival 8 at 30 ± 2 days, moribund state; Weight in JEG-3 cells after vaccination slow upward trend in the first 12 days thereafter decreased weight: Micro CT examination of lung Ministry were not seen obvious placeholder; naked eye, 2/8 subcutaneous observed 5-10mm violet blue metastases to other organs were not observed metastases were not checked to see chest, ascites . D mice weight is always a gradual upward trend over time into. Conclusion: JEG-3 cells by tail vein injection of SCID mice human choriocarcinoma lung metastasis model can be successfully established, 5 × 10 6 cells / ml 0.2ml to create a model of a more appropriate cell concentration, cell number. Preliminary observation of the second part of the SCID mice Intriguring experimental lung metastasis model biological characteristics Objective: To observe the biological characteristics of SCID mice Intriguring experimental lung metastasis model. Method; β-HCG antigen specificity of tumor-bearing lung tissue biopsy immunohistochemistry to detect, fresh tumor-bearing lung tissue in primary culture, the cells were purified and passaged, and note JEG-3-LM cells, the cell-specific antigen β-HCG immunohistochemistry detection and analysis of their chromosomes. Results: biopsy applications Intriguring markers β-HCG stained positive reaction. Choriocarcinoma JEG-3-LM transmitted to the 5th generation, basic removal of fibroblasts, currently passaged for 15 generations; JEG-3-LM monolayer epithelial-like growth, cell morphology was typical epithelioid cells, an average of three four days to generation. Application Intriguring markers β-HCG staining in JEG-3-LM cells all positive reaction. JEB-3-LM karyotype analysis of human-derived monomer, three-body and multi-body chromosome, the chromosome number 19-128, chromosome number 70-79. Conclusion: JEG-3-LM cells are human-derived choriocarcinoma cell. In this study, short people choriocarcinoma cell line JEG-3 research material SCID mice as host animals successfully established lung metastasis model of human choriocarcinoma SCID mice by intravenous injection of tumor cells, and the model of biology The characteristics of a preliminary observation. Currently is yet to be seen in the literature for the host animal, SCID mice to establish experimental of choriocarcinoma lung metastasis model reported by the intravenous injection of tumor cells.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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