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Herbal medicine mainly to the nature of the plants, animals and minerals as raw materials, natural substances is most easily absorbed by the animal, the cows Lactagogue good results, little side effects, almost no residue, no drug resistance, and does not pollute the environment. Therefore, according to traditional Chinese veterinary medicine theory and modern Chinese medicine research to the small the source white natural side effects, no residue, easy to produce green feed additive compound resistant to traditional Chinese medicine as raw material, can be made without antibiotics. Research and Application of cows additives widespread attention. With replenishing qi and blood, through the meridians, reconcile stomach herbal compound preparation has a significant role of prolactin. Codonopsis, Atractylodes, Astragalus, Vaccaria contains a variety of nutrients and bioactive substances, angelica root, loofah, Passepartout, licorice and other traditional Chinese medicine, qi, nourishing, through the meridians, qi and gas line through the effect of milk in the milk to supplement the nutritional animal appetite, promote digestion and absorption of animal, enhance metabolism, improve hematopoietic function and blood circulation of the body, and the killing of microorganisms, enhance immunity, prevention and treatment of disease, thereby improving lactation performance. The experimental select five kinds from human milk side of Chinese herb, widely used Chinese herbal medicine: do not stay in line, a bead, through the grass, dandelion, Loulu. By ordinary Chinese herbal decoction made of a certain proportion of liquid, sterile filtered made drug solution. Take five kinds of liquid cultured mouse mammary epithelial cells in culture medium were added into the expression of β-casein expression, and with blank group processing prolactin group for multiple comparisons. The five kinds of Chinese medicine in vitro mouse mammary epithelial cell proliferation was measured using the MTT assay; using laser scanning confocal microscope comparison Cyclin D1 and Stat 5; using real time RT-PCR method for quantitative detection of β-casein gene expression situation; use of capillary electrophoresis detected β-casein content; liquid is injected directly into the lactating mouse mammary measured in milk yield. The experimental results show that a bead, through the grass and drain Lo mouse mammary epithelial cell proliferation has significantly promote the role (P <0.05); dandelion mouse mammary epithelial cells of proliferation have very significant to promote the role (P <0.01); king not stay no significant role in the proliferation of mouse mammary epithelial cells. Vaccaria the line can be extremely significant with the increase of cyclinD1 expression level (P <0.01); A bead, through the grass and dandelion can significantly with the increase of cyclinD1 expression level (P <0.05); Loulu cyclinD1 expression level is not significant with. Through the grass, dandelion and Loulu can significantly promote the expression of β-casein Vaccaria A bead can be extremely significant to promote the expression of mouse mammary epithelial cell β-casein. The results of the animal experiments the show that the Vaccaria, a bead Loulu can promote the secretion of milk of mice. The preliminary experiments prove that the five kinds of liquid on the in vitro mouse mammary epithelial cell proliferation and promote the expression of β-casein. In this study, from the molecular level to extend a the herbal additives mechanism research.
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