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Study on the Antitumor Constituents from Paris Polyphylla Smith Var. Yunnanensis

Author: WangYu
Tutor: GaoWenYuan
School: Tianjin University
Course: Pharmacognosy
Keywords: Yunnanensis Parispolyside F Parispolyisde G Steroidal saponins Cytotoxic activity
CLC: R284
Type: Master's thesis
Year: 2007
Downloads: 306
Quote: 1
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Abstract


The TCM Paridis Liliaceae yunnanensis Floor (Paris polyphylla Smith var. Yunnanensis (Franch.) Hand.-Mazz.) Polyphylla the drying of the two variants (Paris polyphylla Smith var. Chinensis (Franch.) Hara) rhizomes. Mainly distributed in Sichuan, Yunnan, Guizhou and other places. It's a long history of medicinal and anti-cancer drug has long been used in folk and famous Chinese patent medicine Yunnan Baiyao quarter Desheng snake tablets, Gong blood Ning capsule so drugs. Its main chemical composition of steroidal saponins, plant molting hormone, flavonoids, sterols. Early experiments, the yunnanensis ethyl acetate and n-butanol extract has strong anti-tumor effect. Yunnanensis were looking for bioactive chemical monomer, the systematic chemical composition. This topic using silica gel column chromatography, gel chromatography, preparative thin layer chromatography (PTLC) and preparative high performance liquid chromatography (PHPLC) separation means, right the extracting section polyphylla conducted systematic separation separated from the CPC 73 monomer compound, in addition to the compounds of the fatty acids of the initially identified by 1H-NMR, and further spectrum analysis by 13C-NMR, 2D-NMR and high resolution mass spectrometry method identified the structure of the compound 18, and the remaining compound is identified in. 2 is a new compound: 3 flavonoid glycosides (3-5); one sphingoglycolipid ParispolysideF (1) and ParispolyisdeG (2); (6); an ethyl-alpha-D-fructofuranosidase glucoside (7 ); 1 β-ecdysone (8); 1 stigmasterol-3-O-beta-D-gluco pyranoside (9); 9 steroidal saponins (10-18). Which compounds 3-7 for the first time severely floor isolated in the case of plants, while 3-5 is the species severely floor for the first time assigned to the outside in addition to the previously reported flavonoid kaempferol phenolic glycosides another type of different mouse CINDY LEE class of flavonoid glycosides; compounds 10 and 11 for the first time isolated from this plant steroidal saponins. Yunnanensis extracts in preliminary experiments show, the topic has clear based on the anti-tumor effect, LA795 mouse lung adenocarcinoma cell study investigated the anti-tumor effect of the isolation and identification of compounds were. Studies show that the compounds 1-6 LA795 cells having a certain inhibition, wherein the activity of the new compounds 1 and 2 and the compound 4,10,11 relative better compound 12-18 LA795 cells showed strong cytotoxicity activity. In the the cytotoxicity experiment results on the basis of preliminary discussion of the structure-activity relationship between the cytotoxic activity of the compound structure. The pharmacological results suggest that both diosgenin or partial Connaught saponins have cytotoxicity, and hydroxyl groups on the presence or absence of 17 had no significant effect on the cytotoxicity. Sapogenin C-3 sugar chain is connected, the number of connected location and composition of the type of sugar on its biological activity plays a vital role.

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