Dissertation > Excellent graduate degree dissertation topics show

Study of the Antitumor Activity and the Mechanisms of Phycoerythrin from Gracilaria Lemaneiformis

Author: XuBenJie
Tutor: ChenMeiZhen
School: Shantou University
Course: Biochemistry and Molecular Biology
Keywords: Asparagus Phycoerythrin Antitumor Effect of Immunization p53
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 148
Quote: 0
Read: Download Dissertation

Abstract


In this paper, Shantou South Australian waters the asparagus (Gracilaria Lemaneiformis) the phycoerythrin (Phycoerythrin, PE), anti-tumor activity. Through the establishment of a tumor mouse model to study phycoerythrin 22 anti-tumor effect of hepatoma cells H phycoerythrin H 22 tumor-bearing mice immune function and to explore influence and impact of tumor gene expression of p53, tumor cells cultured in vitro H 22 inhibition of the growth of the tumor suppressor gene p53 expression, in order to reveal the anti-tumor mechanism. Mice in vivo anti-tumor Experimental results show that: PE, given by gavage, the inhibition rate of 30.23% when the dose is 300 mg / kg · d; tumor-bearing mice erythrocyte superoxide dismutase (SOD ) activity and liver homogenates lipid peroxidation (LPO) Determination results show that PE has a role to improve the antioxidant capacity; well protected PE immune organs such as the thymus, spleen, PE can improve the tumor-bearing mouse spleen lymphocyte proliferative responses to ConA, enhanced macrophage phagocytic activity and cytotoxicity of NK cells and red blood cells of the tumor cell adhesion and promote the secretion of TNF-α. Using semi-quantitative RT-PCR method of PE H 22 of p53 and Caspese-3 in tumor-bearing tumor mice tumor tissue impact of the level of gene transcription. The results showed that the PE can promote tumor suppressor gene p53 and apoptosis-related proteolytic enzymes Caspese-3 gene expression in the tumor tissue, to promote tumor suppressor gene expression in vivo PE, thereby inhibiting tumor growth, or even leading to tumor cell withered perish. Cell growth curve assay the PE 22 tumor cells in vitro H , results showed that PE has no inhibitory effect on H 22 tumor cells. The test results suggest that the PE has anti-tumor activity is not directly on the H 22 cells, may be through the promotion of the tumor tissue in the tumor suppressor gene p53 and apoptosis-related proteolytic enzymes Caspese-3 gene expression, improve immunity and antioxidant effects can be achieved. The article also discussed the immunological activity of asparagus polysaccharides (PGL), cyclophosphamide establish immunocompromised mice model (IDMM) to study the role of PGL IDMM immune regulation. The results show that, PGL has to increase the role of immunity in immunodeficient mice, but this effect is immeasurable effect relationship, no direct contact with the polysaccharide contained sulfate groups.

Related Dissertations

  1. The Regulate and Controlof Slug on P53-MDM2 System,R730.2
  2. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  3. Research on Biological Modification and Protection Activity of R-Phycoerythin from Porphyra Yezoensis,TS201.21
  4. Research of Screening and Function of P53 Regulators in KZNF (AxZ Type、SABZ Type and ACxZ Type) Family,R730.2
  5. The Expression of PI3K and P53 in Prostate Cancer and Its Clinical Significance,R737.25
  6. Protein Expressions of EGFR、K-ras、P53 and Ki-67 in Colorectal Cancer, Gastric Cancer and Esophageal Cancer,R735
  7. Research of Expression and Clinical Significance of p53、PCNA and PTEN in Gastric Cancer,R735.2
  8. Expression of hPOT1、p53 and Their Relationship with HP Infection in Gastric Carcinoma,R735.2
  9. Wheat germ extract antioxidant, anti- tumor activity of,R151
  10. Studies on the Low-cost Cultures of Porphyridium Cruentum and Purification of Phycoerythrin,Q943.1
  11. Expression of WWP1 and Its Correlation with ER, CerbB-2 and P53 in Breast Invasive Ductal Carcinoma,R737.9
  12. PUMA, P-gp and GST-π in cervical squamous cell carcinoma patients before and after neoadjuvant chemotherapy tissues,R737.33
  13. The Expression of Topo Ⅱα,P53 Protein in Different Molecular Subtypes of Breast Cancer and Their Prognostic Values,R737.9
  14. The Expression of FHIT, P53 and Ki-67 in Bladder Transitional Cell Carcinoma and the Relationship between Them,R737.14
  15. Expression and Clinical Significance of WWOX and P53 Gene in Colorectal Carcinoma Tissues,R735.3
  16. The Expression and Clinical Significance of B7-H4, OPN and P53 in Epithelial Ovarian Tumors,R737.31
  17. Research of Monocyte Chemotactic Proteins-1 Serum Levels in Breast Cancer Patients,R737.9
  18. Expressions and Clinlcal Significances of P53 in Triple-negative Breast Cancer,R737.9
  19. The Expression and Relational Study of P53, MDM2 and PTEN Protein in Human Oral Lichen Planus and Human Squamous Cell Carcinoma,R739.8
  20. The Expression of Programmed Cell Death 5 and P53 Protein in Mycosis Fungoides of Different Stages,R739.5
  21. Radioprotective Effect and Mechanism of Nitroxides R-1,R965

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile