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The ginseng the green production processing and quality control technology research

Author: ZhangYuChi
Tutor: WangShuMin；LiuZhiQiang
School: Changchun University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: The plasma seed processing technology Bio-organic fertilizer Red ginseng Ginseng Ginseng preserved fruit Processing Liquid chromatography - mass spectrometry technology Fingerprinting
CLC: R283
Type: Master's thesis
Year: 2008
Downloads: 310
Quote: 1
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Abstract


Plasma seed treatment technology ginseng saponin content analysis method. Ginseng extract using an ultrasonic method, UV - visible spectrophotometric analysis of plasma ray total ginsenoside content. Monomer saponin content using high performance liquid chromatography and electrospray ionization mass spectrometry observed plasma ray. The results found that the plasma treatment techniques to promote the role of ginseng ginsenoside content. The total saponin content normally distributed, with the the magnetized coil current strength enhanced current intensity of 1.1 ~ 1.5A TSPG highest content. Establishment of the evaluation method of the of green ginseng production process quality. The use of UV - visible spectrophotometry and high performance liquid chromatography TSPG and monomer saponin content analysis. Experimental results show that the application of organic manure cropping agent with no organic fertilizer the cropping to compare, in the application of organic manure cropping combined cropping agent role of ginseng improved cropping to. Ginseng deep processing, processed red ginseng, ginseng, ginseng preserved fruit ~ -, and three processed products were detected. Using Orthogonal Design red ginseng processing. The results showed that when the conditions for the steaming time of 2min, 30min, the temperature rose from 20 ℃ to 100 ℃, baking temperature of 60 ° C under the conditions of processing red ginseng total saponin content, beautiful color. Improved ginsenoside extraction conditions and to optimize the conditions of liquid chromatography with mass spectrometry, liquid chromatography - electrospray ionization mass spectrometry (HPLC-ESI-MS) spectra, Redder participation ginseng ion current of more than 40 kinds Saponins, initially identified the 10 acetylation ginsenoside rarely been reported in the literature: ginsenoside Re, acetylation the acetylation ginsenoside Rd and the stars of the same of the two isomers (MW = 988), ginsenoside RS1 ginsenoside Rs2 its isomers (MW = 1120). By high-performance liquid chromatography with mass spectrometry can visually compare the difference in red ginseng. In source CID tandem mass spectrometry with ion flow graph peak time can identify isomers molecular ion flow peak. The processed ginseng preserved fruit bright color, fresh taste and high saponin content of 2%, and preserved fruit easy to preserve. Ginseng uniform color, moderate hardness, with Ginseng unique aroma. Saponins almost no loss of processing saponin content of 3%, the same as with ginseng, and easy to save. By high-performance liquid chromatography with mass spectrometry combined with technical analysis the ginseng saponins changes before and after the heat treatment, the results show that the ginseng in the process of high-temperature processing, ginsenoside Re, Rg1, Rf, Rb1, Rg2, Rc, Rb2, Rd, Ro, reduced, generating very little content ginseng ginsenoside Rg6 At F4, Rk3, Rh4, 20S-Rg3, 20R-Rg3 Rk1, Rg5 ginsenoside structure analysis by mass spectrometry technology, the principle of reaction. Ginsenoside 20 glycosyl instability of the tertiary carbon, Ginseng Saponins 20 in the high temperature treatment in the process linked glycosylation hydrolysis occurs, and accompanied by the dehydration reaction, was generated only in the 3 or 6 linked glycosylation secondary saponins, in generate secondary saponins while unsaturated ginsenosides. The fingerprint technology plays an increasingly important role in the authenticity of identification of Chinese herbal medicines. Ginseng, American ginseng, Campanulaceae littoralis chromatography and MS Fingerprint using high performance liquid chromatography and electrospray ionization mass spectrometry, the difference by Chinese herbal medicines fingerprints, to achieve the purpose of the identification of Chinese herbal medicines authenticity; particular the establishment of MS Fingerprint method has the advantages of simple, rapid, sensitive, strong characteristic, good reproducibility, and has a very good practical value.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Chinese medicine processing,preparation
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