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Effects of Interleukin-6 and Pioglitazone on Visfatin mRNA Expression in 3T3-L1 Adipocytes

Author: ShiMin
Tutor: WangChangJiang
School: Anhui Medical University,
Course: Internal Medicine
Keywords: Interleukin-6 Pioglitazone 3T3-L1 adipocytes cells Visfatin Insulin Resistance
CLC: R589
Type: Master's thesis
Year: 2008
Downloads: 61
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Abstract


Objective To observe the interleukin -6 (IL-6) and pioglitazone 3T3-L1 adipocytes cells Visfatin mRNA expression . Cultured in vitro 3T3-L1 before the fat cells and inducing their differentiation Mature , respectively, of different concentrations of IL-6 or pyrazolyl grid column ketone intervention extract cells total RNA was in different time periods , using RT-PCR to detect VisfatinmRNA the expression level , in order to determine both optimal dose and time alone . Then differentiated mature of 3T3-L1 fat cells were randomly divided into 5 groups : control group : do not add any intervention agent ; pioglitazone ketone group : alone adding pioglitazone ; of IL-6 group : alone added IL - 6 ; pioglitazone pretreatment groups : first with topiramate grid column ketone pretreatment cell 1 hours removed topiramate grid column ketone and then added IL - 6 ; of IL-6 topiramate grid column ketone group : at the same time adding IL-6 and topiramate grid column one common role ; above each group drugs were to use the optimal dose , the role of the best time observation VisfatinmRNA the expressed. Results IL-6 or topiramate grid column ketone alone intervention differentiation and maturation of 3T3-L1 fat cells Visfatin mRNA in the expression of water on average lower than the blank control group , and when IL-6 concentrations reached 3ng/ml or , pioglitazone rosiglitazone concentration up to 10μmol / L or more when there are significant differences in the expression of . Intervention in the differentiation and maturation of 3T3-L1 adipocytes cells with of pioglitazone preconditioning or IL -6 pioglitazone rosiglitazone increased Visfatin mRNA expression levels of IL-6 alone intervention , but the difference was not statistically significant . Conclusion IL - 6 or pioglitazone rosiglitazone can cut mature 3T3-L1 fat cells Visfatin mRNA expression levels , and the two together or pioglitazone pretreatment failed to improve Visfatin mRNA expression of IL-6 inhibition . Negative regulation of IL-6 Visfatin mRNA expression may be caused by one of the mechanisms of insulin resistance and glucose and lipid metabolism disorders , Visfatin may not thiazolidinedione (TZD) class of drugs to improve insulin sensitivity of target genes .

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