Dissertation > Excellent graduate degree dissertation topics show

Effects of Trimetazidine on Cardiomyocyte Apoptosis in Late Reperfusion after Acute Myocardial Infarction in Canine

Author: XiaMingWei
Tutor: MaLiKun
School: Anhui Medical University,
Course: Internal Medicine
Keywords: trimetazidine acute myocardial infarction late reperfusion apoptosis canine
CLC: R542.22
Type: Master's thesis
Year: 2008
Downloads: 49
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE Reperfusion of acute myocardial infarction（AMI） is the most effective method at present.It has already confirmed that earlier reperfusion of ischemia myocardium can decrease the infarct size,on the other hand the cardiocyte injury may be aggravated,such phenomenon is called ischemia reperfusion injury（IRI）.It has confirmed that late reperfusion of ischemia myocardium also can delay myocardium remodeling and preserve heart function.But some recent research has proved the existence of late ischemia reperfusion injury in border region of infarcted myocardium. Trimetazidine,an antioxidant agent,has been extensively used in coronary artery disease and other cardiovascular disease.Also,it has been shown that trimetazidine can inhibit myocytes from ischemia reperfusion mediated apoptosis.But it is note clear wheather trimetazidine also inhibit myocytes apoptosis from late reperfusion.We investigated the cardiopro-tection effection and the possible mechanism of trimetazidine in the risk areas during late reperfusion after acute myocardial infarct in canine.Methods Twenty-four adult dogs were randomly divided into three groups:sham operation group（n=8）,late reperfusion group（LR,n=8）,and late reperfusion after trimetazidine treatment group（LR+TMZ,n=8）.Physiological saline was orally given in the sham operation group and LR group and trimetazidine(2mg·kg-1·d-1) in the LR+TMZ group for fourteen days.Fourteen days later,all dogs were received chest operation and coronary artery was exposed.The late reperfusion of acute myocardial infarction model was made by ligating the coronary for 10 h,followed by reperfusion of 10h.Apoptotic index were detected by TUNEL.The Bcl-2,Bax,Cytochrome C and Apoptosis-inducing factor（AIF） proteins were detected by immunohistochemistry.Results Compared with LR group,myocardial apoptotic index and the expression of Bax,Cytochrome C and AIF proteins in LR+TMZ were decreased significantly（all P＜0.01）,but the expression of Bcl-2 proteins were increased（P＜0.01）.Comparing with shame operation group respectively,all indicators were significantly different（p＜0.001）.Conclusion Trimetazidine can decrease cardiomyocyte apoptosis in the risk area during late reperfusion after acute myocardial infarction,which may be related to the increased expression level of Bcl-2 proteins and decreased expression level of Bax, Cytochrome C and AIF proteins.

Related Dissertations

  1. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  2. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  3. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  4. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  5. Platelet parameters in patients before and after acute myocardial infarction,R542.22
  6. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  7. The Expression of CPV-2 VP2 Gene in SF9 and Development of Indirect Elisa for Serum Antibodys,S852.65
  8. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  9. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  10. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  11. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  12. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  13. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  14. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  15. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  16. The Establishment and Application of cPL Double Anti-Body Sandwich Elisa Method for the Diagnosis of Canine Acute Pancreatisis,S858.292
  17. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  18. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  19. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  20. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  21. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases > Myocardial infarction
© 2012 www.DissertationTopic.Net  Mobile