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PARP Activation and Plasma Inflammatory Cytokines in ACS
Author: YaoLan
Tutor: LiaoYuHua
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Inflammatory cytokines Immune inflammatory system Mononuclear Cells Cardiac contractile function Poly ADP-ribose synthetase activity
CLC: R541.4
Type: Master's thesis
Year: 2007
Downloads: 28
Quote: 0
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Abstract
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BACKGROUND: 1 type of poly ADP-ribose synthetase (poly (ADP-ribose) polymerase-1, PARP-1) involved in the transcriptional regulation of a variety of inflammatory cytokines . Study of acute coronary syndrome (ACS) patients with peripheral blood mononuclear cells (MNC) in PARP-1 activity helps to explain the peripheral blood of patients with ACS, the phenomenon of increased plasma levels of inflammatory cytokines . Objective: To investigate patients with ACS, peripheral blood mononuclear cells , PARP-1 activity in plasma tumor necrosis factor -α (TNF-α), leukocyte interleukin -10 ( IL -10 ) , leukocyte interleukin -6 (IL-6 ) level relationship . Methods: The study selected patients with acute myocardial infarction (AMI) patients , 46 and 46 , patients with unstable angina pectoris (UAP) , patients with stable angina pectoris (SAP) , 10 healthy controls (Control) 20 . Determination MNC of PARP-1 activity of PARP activity detection kit (universal colorimetric PARP assay kit) ; enzyme-linked immunosorbent assay (ELISA) determination of plasma TNF -alpha , the level of IL -10 and IL-6 ; Determination of Doppler echocardiography in patients with left ventricular ejection fraction (LVEF) and fractional shortening (LVFS). Results : AMI and UAP patients MNC PARP-1 activity was significantly higher than the SAP group and Control group , which AMI72h group PARP - 1 showed the highest activity for 4.08 times the SAP group , Control group 4.67 times , PARP-1 activity no significant difference in the SAP group and Control group . All AMI and UAP groups plasma TNF - α , IL -10 and IL-6 levels significantly higher than those in the SAP group and Control group ; plasma IL-6 levels in AMI 4w when SAP group and Control The group is no obvious difference . The peripheral blood of patients with ACS cycle MNC PARP-1 activity and plasma TNF -α , IL - 10 , IL - 6 levels , and TNF -α / of IL- 10 ratio positively correlated with the patient (blood collection day line Doppler heart ultrasonic testing ) in LVEF and LVFS were negatively correlated . Conclusion: acute coronary syndrome in the peripheral blood of patients with plasma TNF - α , IL -10 and IL-6 levels increased with MNC PARP-1 activation was significantly associated . PARP-1 may be an important regulator of one of the mechanisms of acute coronary syndrome immune inflammatory system activation process .
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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)
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