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The Effect of HBx Gene to the Apoptosis of Hepatic Cells

Author: YeLu
Tutor: QiJunYing
School: Huazhong University of Science and Technology
Course: Infectious Diseases
Keywords: HBx gene Apoptosis Inhibitor of apoptosis protein XIAP Hepatocellular carcinoma
CLC: R735.7
Type: Master's thesis
Year: 2007
Downloads: 101
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Abstract


Background : in recent years, hepatitis B virus (HBV) X gene on hepatocyte apoptosis and its relationship with hepatocellular carcinoma more and more attention has found that HBx can affect hepatocyte apoptosis through a variety of channels , but the exact mechanism is unknown. The inhibitor of apoptosis protein family members XIAP as an important apoptosis suppressor protein plays an important role in cell apoptosis process , this study examines the role of XIAP in HBx affecting the liver cell apoptosis and carcinogenesis process . Objective: To study in HBx gene expression in HepG2 cells and normal liver cell line L02 of apoptosis and inhibitor of apoptosis protein XIAP expression in the cells . Method: 1. Recovery and cultured HepG2 cells and L02 cell lines ; 2 . Construct pcDNA3.1-HBx plasmids were transiently transfected HepG2 and L02 cell lines ; 3. RT-PCR detected in transfected cells , expression of HBx gene ; 4. Realtime fluorescence quantitative PCR detection XIAP gene expression quantitative differences before and after transfection ; immunocytochemistry to detect protein expression of XIAP protein before and after transfection position and differences ; 6 . flow cytometry apoptosis . Results: HBx gene in L02/pcDNA3.1-HBx , and HepG2/pcDNA3.1-HBx cell expression , proved transfection successful ; 2 L02/pcDNA3.1-HBx cell morphological changes by the larger circle into a spindle-shaped, similar to HepG2 cells ; XIAP gene expression is less than 3 L02/pcDNA3.1-HBx cells L02 cells ( P lt; 0.01 ) ; the XIAP gene HepG2/pcDNA3.1-HBx cells express the amount of HepG2 cells ( P lt; 0.01 ) ; XIAP protein expression than not . L02/pcDNA3.1-HBx cells transfected with HBx gene L02 cells less ( P lt; 0.05 ) ; HepG2/pcDNA3.1-HBx cells XIAP protein expression is not transfected with HBx gene HepG2 cells ( P lt; 0.01 ) ; 5 L02/pcDNA3.1-HBx cells apoptosis rate than L02/pcDNA3.1 the cells increased ( P lt ; 0.01) ; HepG2/pcDNA3 The .1-HBx apoptosis rate than HepG2/pcDNA3.1 the cells decreased slightly ( p lt; 0.05 ) . Conclusion : HBx gene can promote apoptosis of normal liver cells , inhibition of apoptosis of hepatoma cells . One of the mechanism of this effect may be inhibition through the regulation of apoptosis proteins XIAP expression .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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