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Honeysuckle plant honeysuckle Caprifoliaceae (Loniccra japonica Thunb), red gland Lonicera (Lhypoglauca Miq), Lonicera (L confusa DC) or hair style honeysuckle (L dasystyla Rchd) dried flower buds, and its sweet, cold, the lung , heart and stomach. Detoxification, dispelling wind-heat, with a pathogenic microorganism, anti-drug, anti-inflammatory and antipyretic effect. At present, for the active constituents of honeysuckle is limited to small molecules, and for the great development prospects polysaccharides few studies. In order to further development and utilization of resources, honeysuckle, honeysuckle selected as the material of the subject, the use of modern technology for honeysuckle polysaccharides were isolated and purified, and the purified fractions were determined composition and structure, the use of modern functional evaluation method polysaccharide extracts of honeysuckle The antioxidant activity in vitro and in vivo antioxidant activity in mice conducted a preliminary study, the test results are as follows: 1. using water-soluble polysaccharide alcohol precipitation honeysuckle extract optimum extraction condition was A 1 B 2 C 1 D 2 , that is 40 ℃, 2h, 20 times of water, extracted two times. 2 ultrafiltration results showed that: more than half of the molecular weight polysaccharide honeysuckle 100KD above ,100-50KD ,50-30KD and 30-10KD range of distribution has honeysuckle polysaccharide. 3 The DEAE-52 cellulose ion-exchange column chromatography and SephadexG-100 gel column chromatography of the FL-C1, FL-D1, FL-D2 three components, were used to identify the purity of Sephadex G-200 gel column chromatography, and high performance gel permeation chromatography, this indicates that FL-C1, FL-D1 two components are single component. Calculated FL-C1, FL-D1 molecular weights of 120000Da, 30000 Da. 4 monosaccharide composition analysis showed, FL-C1 from glucose (Glu), galactose (Ga1), ribose (Rib), arabinose (Ara), xylose (Xy1), fructose (Fru) components. FL-D1 from glucose (Glu), galactose (Gal), xylose (Xyl), arabinose (Ara), rhamnose (Rha) components. FL-D2 from glucose (Glu), galactose (Gal), mannose (Man), ribose (Rib), arabinose (Ara), xylose (Xyl), fructose (Fru) components. 5 In vitro antioxidant activity showed that the total polysaccharide honeysuckle reducing power gradually increased with increasing concentration and the concentration of the polysaccharide has a good correlation. Honeysuckle polysaccharides on PMS-NADH-NBT system, superoxide radicals generated significant inhibition. 6 mice showed antioxidant activity, honeysuckle extract polysaccharides can significantly improve the test serum, liver, brain tissue superoxide dismutase (SOD), catalase (CAT) and glutathione over peroxidase (GSH-PX) activity was significantly decreased malondialdehyde these organizations (MDA) levels. The results illustrate honeysuckle polysaccharide extract has antioxidant activity.
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