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Licorice is a commonly used Chinese herbs with Spleen Qi, detoxification, lungs and cough, urgency and pain relief, to reconcile the effect of various drugs. Excavation of the man-made destructive of wild licorice, licorice protective utilization imminent. Cultured in vitro regeneration system and hair root for the establishment of the technology, licorice resources protection, genetic engineering and the active ingredient use is important. Uralensis, Glycyrrhiza inflata, Glycyrrhiza glabra, yellow licorice and wild Glycyrrhiza glabra, the study for the test material, regeneration and hairy root induction of licorice from the main findings are as follows: 1. Establish a licorice under hypocotyl vitro regeneration system laid the foundation for genetic engineering licorice breed improvement and rapid propagation of superior varieties. To licorice hypocotyls as explants, adventitious buds factors affect hypocotyl regeneration, the results show that, KT has a significant role, with 6-BA, IBA combined could efficiently induce hypocotyl directly produce adventitious buds differentiation medium optimized for the MS 6-BA 2.0 mg / L KT 0.5 mg / L IBA 0.5 mg / L, sprouts differentiation average rate of 41.5%. Glycyrrhiza inflata hypocotyl highest regeneration frequency of 44.7%; preculture 4 days and of AgNO3 6 mg / L of licorice hypocotyl regenerated adventitious buds have a role in promoting. Established licorice rapid propagation technology system, laid the foundation for the further development and use of the excellent licorice varieties. Licorice subband cotyledon stem segments and the the band the leaf stems segment as explants, the results show that: the Ural licorice induction profusion seedlings more three tested materials; cotyledons stem segments liquorice rapid propagation of the most appropriate the explant; licorice rapid propagation system suitable medium: induction medium MS 6-BA 0.5 mg / L KT 0.5 mg / L IBA0.1 mg / L the rejuvenation medium MS 6-BA 2.0 mg / L KT, 0.5 mg / L IBA0.5 mg / L rooting The medium MS IBA0.5 mg/L3. establish licorice embryogenic callus induction and somatic embryogenesis system, for licorice genetic transformation and artificial seed seed material. Uralensis, Glycyrrhiza inflata, Glycyrrhiza glabra, yellow licorice materials via callus induction, embryogenic callus formation and generation of embryoid genotype, explant, hormones, additives, etc. factors research and analysis of the occurrence and development process of embryoids The results show that, licorice embryoid bodies occur in three ways: First, from the upper end of the hypocotyl morphology occur directly, without going through callus phase, but induced embryoid less body; after callus stage somatic embryos differentiate into embryoids induced; occurred from the free cell suspension induced embryoids. Licorice the embryoid three occurred way to differentiate into regenerated plants are more difficult. Embryogenic callus and non-embryogenic callus morphology and structure was further analyzed using paraffin sections and semi-thin sections, found embryogenic callus, embryoids and non-embryogenic callus, embryoid The morphology and structure are quite different. Several suitable medium: callus induction MS 6-BA 2.0 mg / L 2,4-D 0.5 mg / L callus following generation of MS 6-BA 0.5 mg / L NAA 0.2 mg / L 2,4 -D 0.2 mg / L of embryonic callus induction MS 6-BA 0.5 mg / L KT 0.5 mg / L IBA 0.1 mg / L embryogenic callus Following the generation of embryoid body differentiation medium MS 6-BA 0.5 mg / L KT 0.5 mg / L IBA 0.1 mg / L ME 500 mg / L of embryoid proliferator MS 6-BA 0.5 mg / L KT 0.5 mg / L IBA 0.1 mg / L ME 500 mg/L4. establish a licorice hairy root The induction technology platform, the licorice hairy root, which laid the foundation for the production of secondary metabolites of licorice hairy root industrialized. Conducted a systematic study of the factors that affect the induction of hairy root (different strains of different explants, different the bacterium concentration of different infection time) The results show that: the strain LBA9402 easy to induce hairy root uralensis sensitive strains. Hypocotyl, cotyledon and leaf induced hairy roots, hypocotyls highest conversion rate. With the LBA9402 Agrobacterium infection bacterial suspension diluted twice Ural hypocotyl hair root, about 5 ~ 10 min to achieve the best. Induced hairy root tender, white, fine, the growth rate is faster, multi-branched root hairs, was shrubby lost geotropism.
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