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Experimental Research on Influence of Rubbit Epithelial Flap in Epi-LASIK for the Expression of TGF-β1, bFGF and Apoptosis in Corneal Stroma

Author: ChenYanLei
Tutor: GuoHaiKe
School: Southern Medical University,
Course: Ophthalmology
Keywords: Epi-LASIK LASEK Flap Cell proliferation TGF-β1 bFGF Apoptosis
CLC: R779.63
Type: Master's thesis
Year: 2008
Downloads: 33
Quote: 0
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Abstract


Background: Epi-LASIK (epithelial laser in situ keratomileusis mechanical method of laser subcutaneous corneal LASIK surgery) has a good safety, efficacy, predictability and stability. However, compared with LASIK (laser in situkeratomileusis laser in situ keratomileusis surgery), Epi-LASIK postoperative visual acuity slow recovery, postoperative pain, especially haze (haze) higher incidence become The main factors restricting its application. Haze accompanied by the generation of corneal wound healing repair process, in recent years, the research on the repair of corneal wound healing: epithelial cell injury can cause apoptosis of stromal cells; corneal stromal cell apoptosis is the main factor to start the repair process; repair process is accompanied by a variety of cytokines, such as TGF-β 1 , bFGF and other synergies. There are studies that the LASEK corneal epithelial healing from outside to inside the epithelial cell division and proliferation of re-covering. Integrated a number of research results, Epi-LASIK surgery reserved flap may cause apoptosis of stromal cells to start one of the factors in corneal wound healing and repair process, Epi-LASIK flap in the corneal wound healing repair process. the role played worth further exploration. The first part of Epi-LASIK, LASEK corneal flap cell culture and proliferation were detected to explore laser subepithelial keratomileusis (laser-assisted subepithelialkeratomileusis LASEK) and mechanical method laser subepithelial keratomileusis (epithelial laser in situkeratomileusis in Epi-LASIK) corneal epithelial healing way. Methods line LASEK and Epi-LASIK corneal flap 20 cases, each rabbit corneal flap of the 12 cases, and the rabbit limbal tissue in 12 cases, using cell and tissue law on the flap cells and limbal cell culture, and used to detect and compare the cell proliferation ability of soluble thiazole salt (WST-8) Act. Using SPSS11.5 software applications independent samples t-test and one-way analysis of variance of the data were statistically analyzed. Results and rabbit flap cells observed continuously for two weeks, only to see the growth of a small number of cells or no cell growth, people the LASEK group of cell proliferative capacity than Epi-LASIK group, the difference between the two groups was statistically significant (t = 3.907, P = 0.000), rabbit the LASEK group of cell proliferative capacity than Epi-LASIK group, the difference was not significant (P = 0.255), the rabbit of LASEK a , Epi-LASIK b group cell proliferation below the limbus group and the differences were statistically significant (P a = 0.000, P b = 0.005). Conclusion flap cell proliferative capacity in vitro on low and the LASEK flap proliferation ability than Epi-LASIK LASEK and Epi-LASIK corneal wound healing process does not rely on the corneal flap, from the angle of cell proliferation In-situ growth, but led limbal stem cell proliferation. The second part rabbit Epi-LASIK flap the the corneal stroma TGF-β 1 of bFGF expression and apoptosis in Objective To compare the rabbit removal on the flap Epi-LASIK and retention flap for the Transforming growth factor-β 1 (TGF-β 1 ), basic fibroblast growth factor (bFGF) in the different expression of the corneal stroma and stromal cell apoptosis level explore flap of corneal wound healing process. Methods 45 New Zealand white rabbits, three as a negative control, 21 rows of traditional Epi-LASIK reserves flap, removal of more than 21 rows Epi-LASIK flap. Respectively after 4 hours, 1, 3, 5 orders, 7, 15, 30 each group were 6, by immunohistochemical method and terminal transferase-mediated dUTP nick end labeling (terminal deoxynucleotidyl transferase-mediateddUTP nick-end-labeling, TUNEL), the corneal stroma TGF-β 1 , bFGF in expression and stromal cell apoptosis level detection and to observe corneal epithelial healing and the occurrence and extent of the haze. Using the the SPSS11.5 software application x 2 test, independent samples t-test and repeated measurement data variance analysis of data for statistical analysis. Results remain flap group keratocytes TGF-β in 1 , bFGF expression and stromal cell apoptosis levels were higher than the flap group, two sets of stromal cell apoptosis level difference was statistically significant (F = 5.452, P = 0.042), the corneal stroma TGF-β 1 a , the expression levels of bFGF b difference was no statistically significant (F a = 0.552, P a = 0.475; F b = 1.602, P b = 0.234); stay flap group compared to the flap Leader epithelial healing time, the difference was not significant (t = 0.640, P = 0.526); Both groups were on the 3rd postoperative a haze after 5 days, 7 days, haze incidence the elevated postoperative haze 15th alleviate postoperative 30 haze disappear, which after 3 days stay flap group haze the high incidence to go flap group, the difference was statistically significant (x 2 = 4.269, v = 1, P = 0.039). Conclusion Epi-LASIK flap in situ reserves the right to increase the degree of stromal cell apoptosis levels and the incidence of postoperative haze, the flap is a negative factor in corneal wound healing and repair process.

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CLC: > Medicine, health > Ophthalmology > Eye surgery and surgery > Retinal photocoagulation and laser eye surgery
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